基于16S rRNA基因的食源性致病菌实时荧光定量PCR检测研究

摘要

本研究以16S rRNA基因作为靶基因,利用TaqMan探针技术建立了针对志贺氏菌(Shigella)、沙门氏菌(Salmonella)和金黄色葡萄球菌(Staphylococcus aureus)三种主要食源性致病菌的多重实时荧光定量PCR检测技术.结果表明,建立的荧光定量PCR检测体系特异性良好、灵敏度高、简便快捷,大大缩短了检测时间,在食源性致病菌快速筛查方面具有良好的应用前景.%In this study, a multiplex real-time quantitative PCR detection technology for the three food-borne pathogens, Shigella, Salmonella and Staphylococcus aureus, had been established utilizing the TaqMan probe and 16S rRNA gene as a target gene.The results indicated that the established multiplex real-time quantitative PCR detection system had good specificity and sensitivity, was simple and efficient, and greatly reduced the detection time, so it had a good prospect in the rapid screening for food-borne pathogens.