为研究四氢嘧啶对凝乳酶热稳定性的影响,向凝乳酶溶液中加入0.175 ～5.600 mmol/L的四氢嘧啶,测定不同浓度四氢嘧啶对凝乳酶活力的影响;之后测定了含0.50 ～2.00 mmol./L四氢嘧啶的凝乳酶溶液在69℃保温不同时间后的凝乳酶活力,将热保温后的酶溶液在4℃复性36 h,并再次测定酶活力的变化.结果显示,不同浓度四氢嘧啶对凝乳酶活力影响不大,各组酶活力均降低8.2％左右;加入四氢嘧啶会显著降低凝乳酶的热稳定性,但会发生明显的复性作用,提示四氢嘧啶可能具有维持部分失活态凝乳酶稳定性的作用.%To study the effect of ectoine on the thermostability of rennin,0.175 ～ 5.600 mmol/L ectoine were added into rennin solution to determine the effects of ectoine concentration on rennin activity,and then the rennin solutions added 0.50 ～2.00 mmol/L ectoine were heated in water bath at 69℃ for different time,and renaturated at 4℃ for 36 hours.The changes of rennin activities after water-bath and renaturation were both determined.The results showed that different concentrations of ectoine had little effects on rennin activity,and the rennin activity decreased by about 8.2％.When ectoine was added into rennin solution,the thermostability of rennin decreased and renaturation would significantly happened,which indicated that ectoine might be essential for keeping the stability of partial inactivated rennin.
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