SB203580对受照小鼠免疫细胞的作用研究

摘要

Objective : To observe the effects of p38 inhibitor SB203580 (SB) on the immune cells in the radiated mice. Methods : C57BL/6 mice were divided into control group, irradiated group and SB group. The control group received sham irradiation ,and the rest accepted 4Gy total body irradi8tion , dose rate 0. 8Gy/min.SB was intraperitoneally injected 15mg/kg at1, 3, 5, 7, 9 day after irradiation. After 4Gy irradiation 10 days,the peripheral blood was collected by eyeballs enucleation. Then the immune cells phenotypes in peripheral blood was analyzed by flowcytometry. Results : Compared to irradiation group, WBC numbers increased and ROS levels of bone marrow cells decreased in the SB group (P＜ 0. 05). Conclusion: The p38 inhibitor SB can decrease the ROS levels of bone marrow cells,has no obvious protective effects on the WBC after 4Gy irradiation.%目的:探讨p38抑制剂SB203580(SB)在免疫细胞辐射损伤中的作用.方法:将C57BL/6小鼠分为对照组、照射组、SB组.对照组接受假照射,对其余两组进行4Gy全身照射.SB203580 15mg/kg腹腔注射,照射24h后开始给药,隔日给药共给药5次.SB末次给药24h后处死小鼠,取外周血进行分型CD4、CD8、B220检测,取骨髓测定ROS.结果:SB组与照射组比较,外周血白细胞增加,骨髓ROS水平与照射组比较有所下降(P<0.05).结论:p38抑制剂能降低受照小鼠骨髓细胞的ROS水平,对4Gy照射后的外周血无显著保护作用.