MP-DNA荧光定量聚合酶链反应检测在儿童肺炎支原体肺炎中的诊断价值

摘要

Objective : To explore diagnostic evaluation of sensitivity and specificity of MP-DNA,MP-Ig are CRP in children With MP pneumonia, and provide accurate and quick diagnostic proof to clinic medicals. Methods: children's respiratory secretion samples were collected for fluorescent quantitative PCR for MP-DNA, and the serum, s concentrations of CRP and MP-IgM were detected by immunobiochemistry methods, and the three indexes were compared with each other. Results: MP-DNA and MP-Ig were superior for sensitivity in diagnosis of MPP. Of the three indexes the MP-Ig was the most sensitive, and MP-DNA was more superior than others in specificity and positive predictors ; negative predictors and Youden' s index of MPDNA and MP-Ig have little difference in common. Conclusion: MP-DNA is evaluable in early and accurate diagnosis on MP infection.%目的:探讨肺炎支原体DNA(MP-DNA),支原体抗体(MP-Ig),C反应蛋白(CRP)对诊断支原体肺炎的敏感性和特异性,为临床提供准确快速的诊断依据.方法:采用MP荧光定量PCR法检测患儿呼吸道分泌物标本中的MP-DNA水平,同时检测血清CRP和MP-IgM的浓度,同时相互进行比较.结果:MP-DNA和MP-Ig对诊断支原体肺炎的敏感性均高于CRP,三个指标中敏感性以MP-Ig最高;特异性和阳性预测值以MP-DNA最高;阴性预测值和约登指数,MP-DNA和MP-Ig两者相差无几.结论:MP-DNA检测对肺炎支原体的早期诊断有一定价值.