缺氧诱导因子-1α在胆囊癌组织中的表达及临床意义

摘要

Objective :To detect the expression of HIF‐1α(hypoxia inducible factor lα) in the tissue of gall‐bladder carcinoma and to evaluate its clinical significance .Methods :HIF‐1α was examined in 30 patients of primary gallbladder carcinoma and in 18 patients suffering cholecystitis respectively using Immunohistochemistry technology , and then analyze the relationship between clinico‐phathological factor of gallbladder carcinoma and the expression of HIF‐1 .Results :The positive rate of HIF‐1 in gallbladder carcinomas was 63 .3% (19/30) .In 18 cases of cholecysti‐tis ,no HIF‐1αexpression was detected .The positive rate of HIF‐1 in gallbladder carcinomas was significantly higher than that in the tissues of cholecystitis (P < 0 .01) .HIF‐1 protein expression was closely relevant with lymphatic metastasis(P = 0 .043) ,TNM stage(P = 0 .021)and tumor size(P = 0 .028) while was negative correlations with prognosis((35 .0% vs 16 .7% ,P= 0 .037) ,but uncorrelated with the pathological grading ,the age and sex of patients (P> 0 .05) .Conclusion :High expression of HIF‐1 plays a considerable role in development of gallbladder carcino‐ma .HIF‐1 could possibly become a helpful marker for diagnosing and assessing prognosis in the carcinoma of gall‐bladder .%目的：探讨缺氧诱导因子（HIF‐1α）在胆囊癌组织中的表达及其临床意义。方法：选取30例胆囊癌组织作为观察组，18例正常慢性胆囊炎组织作为对照组，对观察组和对照组分别利用免疫组织化学技术来检测石蜡病理切片组织中 HIF‐1α的表达情况，进一步分析 HIF‐1α与胆囊癌临床病理因素及预后之间的关系。结果：HIF‐1α蛋白在30例胆囊癌组织中的阳性表达率为63．3％（19／30），而在18例胆囊炎组织中均未见表达，两者间有极显著性差异（P＜0．01）。 HIF‐1α表达与肿瘤 TNM 分期（P＜0．05）及淋巴结转移以及肿瘤的大小呈正相关（P ＜0．05），而与胆囊癌组织分化程度、患者的年龄和性别等无关（P ＞0．05），与预后呈负相关（（35．0％ vs 16．7％，P ＜0． 05）。 结论：HIF‐1α的在胆囊癌的发生发展过程中起着重要作用，可作为判断胆囊癌诊断及预后的有用指标。