布地奈德对哮喘大鼠气道重塑及气道平滑肌中Toll样受体4表达的影响

摘要

Objective :To explore the influence of budesonide on the airway remodeling and TLR4 of airway smooth muscle cell in Rat models of asthma .Methods :Thirty SD rats were randomly divided into 3 groups :model group(group A) ,normal control group(group B) ,budesonide treatment group(group C) ,with 10 rats in each group . The airway inflammation and the alteration of airway structure were detected by HE staining ,The airway wall thick‐ness(WAt/ Pi) ,the bronchial smooth muscle thickness(WAm/Pi)and numbers of smooth muscle cell (N /Pi )were measured by using image analysis system .the expression of TLR4 protein in cells were detected by Western blot .Re‐sults :Bronchial epithelial desquamation ,inflammatory cell infiltration ,hyperplasia of goblet cells and smooth muscle cells were seen in group A under optical microscope ,however compared with group A ,the manifestations were signif‐icantly decreased in group C .Compared with group B ,WAt/ Pi ,WAm/Pi and N/Pi were expressed at higher levels in lung tissues of group A(P < 0 .01) .Compared with group A ,WAt/ Pi ,WAm /Pi and N /Pi were expressed at lower levels in lung tissues of group C(P< 0 .01) .Compared with group B and C ,the expression of TLR4 at protein levels in group A was significantly highter .Conclusion :The airmay remodeling can be inhibited by budesonide proba‐bly through reducing the expression of TLR4 .%目的：探讨布地奈德对哮喘大鼠模型气道重塑及平滑肌中 Toll 样受体4表达的影响。方法：将30只 SPF 级 SD 大鼠随机分为支气管哮喘组（A 组）、健康对照组（B 组）、布地奈德治疗组（C 组）3组，每组10只 。 制备大鼠肺组织病理切片，HE 染色后观察各组气道结构改变情况，并测定支气管壁厚度（WAt／Pi）、支气管壁平滑肌厚度（WAm／Pi）及支气管平滑肌细胞核数（N ／Pi），利用 Western‐Blot 方法检测气道平滑肌中 TLR4的蛋白表达。结果：光镜下可见 A 组支气管上皮细胞脱落、管壁炎症细胞浸润、杯状细胞增生、基底膜和平滑肌明显增厚，而 C 组上述改变较 A 组明显减轻。与 B 组比较，A 组支气管管壁厚度、平滑肌厚度及平滑肌细胞核数显著升高（P ＜0．01），与 A 组比较，C 组支气管管壁厚度、平滑肌厚度及支气管平滑肌细胞核数显著降低（P ＜0．01）。 A组的 TLR4的蛋白水平明显高于 B 组及 C 组（P＜0．01）。 结论：吸入布地奈德可以防治气道重塑，可能通过抑制 TLR4的表达实现。