免疫比浊法测定D-二聚体抗脂浊能力

摘要

Objective:To analysis immune turbidimetric method determination of plasma D-dimer levels and the anti-lipid turbidity capability of D-dimer by immunoturbidimetry ,in order to understand the characteristics and blood lipid in the determination of the degree of interference .Methods:According to NCCLS documents ,use correct experiment ,plasma sample determination and anti-lipid turbidity experiment on the immune turbidimetric method determination of D -dimer . Results :When the D-dimer levels <1 .5μg/ml ,the difference between detection mode D/C and detection mode D/3 are statistically significant difference (t=8 .46 ,P< 0 .05);chylous turbidity of 1200 has negative interference on the immune turbidimetric method determination of plasma D-D ,when turbidity within 1200-4800 ,the scope of the negative interference show a linear trend .Conclusion:The immune turbidimetric method determination of D-dimer has a good effect ,but if tur-bidity chyle is larger in sample ,we should fully take into account the effects of hypertriglyceridemia on the test results and take measures to ensure the accuracy of the correction .%目的：分析免疫比浊法测定D-二聚体及其抗脂浊的能力，了解该种方法的特点和脂血在其测定中的干扰程度。方法：根据NCCLS文件，对免疫比浊法测定D-二聚体进行正确性实验、血浆样本测定和抗脂浊实验。结果：在D-二聚体水平＜1．5μg／ml时D／C、D／3两种检测模式结果具有统计学差异（ P〈0．05）；乳糜1200浊度对D-D浓度的免疫比浊结果具有负干扰且浊度在1200～4800范围内其负干扰成一次线性趋势。结论：免疫比浊法测定D-二聚体具有较好的效果，但在样本乳糜浊度较大时应充分考虑到甘油三酯对检测结果的影响并采取措施进行校正以保证结果的准确性。