柴胡注射液对人肝癌细胞株SMMC-7721增殖及凋亡的影响

摘要

目的：探讨柴胡注射液对人肝癌细胞株SMMC-7721凋亡的影响及相关机制。方法：将人肝癌SMMC-7721细胞株（由西安交通大学医学院第一附属医院临床分子试验中心提供）分为空白对照组（加10％小牛血清的1640培养液）、氟尿嘧啶阳性对照组（10μg／ml）及实验组（分别为5mg／ml、10mg／ml、20mg／ml、25mg／ml、50mg／ml柴胡注射液）。采用噻唑蓝比色法（即MTT）检测人肝癌细胞株SMMC-7721对柴胡注射液药物和氟尿嘧啶注射液的敏感性；应用流式细胞术（FCM）检测柴胡注射液对人肝癌细胞株（SMMC-7721细胞）周期的影响。结果：柴胡注射液对SMMC-7721的生长有明显的抑制作用，抑制率与柴胡注射液浓度呈正相关。不同浓度柴胡注射液处理肝癌细胞时，药物浓度与凋亡细胞且呈剂量依赖关系。结论：柴胡提取物可抑制人肝癌SMMC-7721细胞增殖，诱导其凋亡，作用机制可能与调控细胞周期有关。%Objective :To investigate the effect of bupleurum injection on human hepatic carcinoma cells '(SMMC-7721) apoptosis and related mechanisms .Methods : With different concentrations (0 ,5 ,10 ,20 ,25 , 50mg/ml) treatment liver cancer SMMC-7721 cell line ,determined by M TT method in different concentrations of tumor cells after treated by inhibiting rate ;FCM detect different concentrations of radix bupleuri injection effect on SMMC-7721 cell cycle .Results :Different concentrations of radix bupleuri injection processing SMMC-7721 cell , number of living cells ,cancer cells function inhibition ,inhibition rate and radix bupleuri injection concentration were positively correlated ,when the concentration of radix bupleuri injection of 50mg/ml ,inhibition rate reached the maximum .Radix bupleuri injection 5mg/ml ,10mg/ml ,25mg/ml ,50mg/ml dealing with cells ,apoptosis cells re-spectively accounted for 13 .62% ,15 .32% ,23 .32% ,30 .42% of the total ,apoptosis rate increased with the in-crease of drug concentration ,and a dose-response relationship .Conclusion :Bupleurum extract can inhibits human hepatic carcinoma SMMC-7721 cells'proliferation ,induce its apoptosis ,and the mechanism of action may be related to the regulation of cell cycle .