大豆质核互作雄性不育系W 931 A及其保持系不同器官的可溶性蛋白的电泳分析

摘要

Objective:The paper was aimed at the comparative proteome analysis of different organs between the cytoplasmic-nuclear male-sterile line W931 A and its maintainer W931 B in soybeans to obtain some important information. MethoT: the SDS-PAGE. Result: The SDS-PAGE gel electrophoresis maps showed that no protein spots were found differentially expressed between the male-sterile line W931 A and its maintainer W931 B seeds or leaves, but 3 protein spots were found differentially expressed between W931 A and W931 B anthers at binucleate pollen stage. Conclusion: The above results inferred that expression of the male sterile genes was spacially and periodly, and the anther was the major organ in which the protein related to the male sterility was expressed.%目的:对大豆质核互作雄性不育系W931 A及其同型保持系W931 B不同器官的蛋白质组进行比较性研究,获得差异蛋白质组的重要信息.方法:采用SDS-PAGE方法.结果:发现不育系W931 A与其保持系W931 B种子和苗期叶片的蛋白质SDS-PAGE电泳图谱基本没有差异带,二胞花粉期花药SDS-PAGE图谱间有3条差异带.结论:雄性不育基因表达具有时空和器官特异性.与育性有关的蛋白主要在花药中表达.