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山药零余子对多效唑的响应部位和化感作用研究

     

摘要

In order to study the sensitive section of yam bulbils to paclobutrazol ( PP333 ), different concentration(10 mg/L,100 mg/L and 1 000 mg/L) of PP333 were stabbed into the top, the middle and the base of bulbils with a needle, then the effect of different treatments on the germination rate was studied. The germination rate of Chinese cabbage seeds treated with the extract from the base and the top of dormant bulbils and germinating bulbils was tested,so as to find whether restricting matters in the bulbils. The results showed that the germination rate of the bulbils treated at the base and the top was same as the control. The bulbils treated at the middle were promoted to germinate, and the more concentrated PP333 solution the better the effect. The extract from neither dormant bulbils nor germination bulbils restrained Chinese cabbage seeds germination,the surprising result was that the extract from the base of dormant bulbils promoted the seeds germinating extremely significantly. The results indicate that bulbil dormancy did not result from the restricting germination matter,stabbing bulbils with PP333 promoted the bulbil germinating,and the sensitive part of bulbil to paclobutrazol was the middle part.%用10、100、1000 mg/L的多效唑针刺处理山药余零子基部、中部及顶部,研究零余子对多效唑感应的部位差异.用休眠期和萌芽期的零余子浸提液处理大白菜种子,再检测大白菜种子的发芽率,以检测零余子中是否含有抑制发芽的物质.结果表明,多效唑处理零余子基部和顶部不能促进零余子发芽,处理中部能促进发芽,在10~1000 mg/L的浓度范围内其促进效果随多效唑浓度增加而加强.休眠期和萌芽期的零余子基部和项部都不存在抑制大白菜种子发芽的物质,休眠期零余子基部浸提液能极显著地提高大白菜种子发芽率.以上结果说明,山药零余子休眠不是因抑制生长的物质引起,用多效唑针刺处理能促进零余子发芽,多效唑针刺处理的有效部位是零余子中部.

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