外源供体硝普钠对干旱胁迫下大豆幼苗游离脯氨酸及抗氧化酶的影响

摘要

In order to explore the effect of sodium nitroprusside (SNP) under drought stress,effects on the activities of free proline,SOD and catalase from soybean seedlings were studied.Experimental results show that:when the concentration of SNP was between 0-90 μmol/L,the content of proline,superoxide dismutase (SOD) and catalase increased.When the concentrations of sodium nitroprusside was 90μmol/L,the content of proline,superoxide dismutase (SOD) and catalase reached the maximum.When concentration of SNP was between 90-150μmol/L,the content of proline,superoxide dismutase (SOD) and catalase decreased.So when the concentration of SNP was 90μmol/L,it worked best.Soybean was the research object in this experiment,to explore the effects of exogenous sodium nitroprusside on soybean seedling growth under drought stress,to find the optimal concentration of sodium nitroprusside under drought stress.%使用20％ PEG-6000进行干旱模拟,通过测量大豆幼苗中的游离脯氨酸含量、超氧化物歧化酶活力及过氧化氢酶活性的变化来分析硝普钠对大豆抗旱的影响.结果表明:当硝普钠浓度在0～90μmol/L之间时,游离脯氨酸含量、超氧化物歧化酶活力、过氧化氢酶活性等3项生理指标均随之升高,当硝普钠浓度为90 μmol/L时,3项生理指标达到最大值,当硝普钠浓度在90～150μ mol/L之间时,3项生理指标又随着硝普钠浓度的升高而降低.所以,当硝普钠浓度为90 μmol/L时,对大豆幼苗中游离脯氨酸含量、超氧化物歧化酶、过氧化氢酶影响最大,为促进作用.本实验以大豆为研究对象,探讨外源供体硝普钠对干旱胁迫下大豆幼苗生长的影响,旨在了解硝普钠缓解干旱胁迫的最佳浓度.