宁强矮马生长激素基因(GH)的遗传变异分析

摘要

[目的]通过分析不同动物和中国几个地方马种生长激素基因序列,为宁强矮马起源和矮化形成机制的研究提供分子水平的试验依据.[方法]通过PCR扩增宁强矮马生长激素基因并进行测序,用DNAMAN软件对不同动物的生长激素基因序列进行比对分析.[结果]马品种问29个位点发生遗传变异,编码区11个位点发现遗传多态现象.以蒙古马生长激素氨基酸序列为标准,宁强矮马仅在1 765位点的变异导致氨基酸发生改变.以生长教素的mRNA序列为基本数据进行聚类分析,宁强矮马和德保马67的同源性为100%,与百色马的同源性为99.8%,与蒙古马的同源性为99.7%.[结论]马属动物生长基因序列与猪和狗的同源性较高,宁强矮马1 765位的G→A可能影响到宁强矮马的生长发育.%[Objective] In order to provide a scientific basis for the origin and dwarfish mechanism of Ningqiang pony,the sequence of GH of different animals and some China horse breeds were studied,[Method] The PCR amplification,sequencing and DNAMAN software were used.[Result]Them were 29 genetic variation,11 loci were found in the coding region.Taking the AA sequence of GH of Mongolia horse as standard,there was 1 amino acid variation in Ningqiang pony.Through clustering analysis based on the sequence of mRNA of GH,the homology was 100%,99.8%,99.7%,between Ningqiang and Debao 67,Baise and Mongolia.[Conclusion]The homology level of GH was higher among Equus caballus,Sus scrofa and dog in present study,and the mutation of G→A affects the growth of Ningqiang pony.