Polimorfisme DNA pada Lokus-2 Gen Hormon Pertumbuhan Sapi Madura ud udDNA polymorphism at locus-2 of growth hormone gene of Madura cattle

摘要

The objectives of the research were to detect DNA polymorphism at locus 2 of bovine growth hormone gene of Madura udcattle and to know its genetic diversity. DNA polymorphisms and their effect on phenotypic traits have been studied widely udin dairy cattle but not for beef cattle, especially for Indonesian local cattle. Polymorphism was detected using PCR-RFLP udusing primer GH-5 and GH-6 for amplifying locus 2 of growth hormone gene. Genetic diversity was analyzed based on the udformula of Nei (1973, 1975). DNA polymorphism was found on locus 2 of growth hormone gene using MspI restriction udenzyme. This polymorphism may be caused the lost of restriction MspI site. The genetic diversity was 0.4422. udud udKey words: bovine growth hormon gene, PCR-RFLP, polymorphism, Msp I restriction enzyme, Madura Cattle.