5-HT及5-HT1A受体在不同妊娠阶段大鼠子宫中的分布

摘要

[目的]研究5-羟色胺(5-hydroxytryptamine,5-HT)及5-HT1A受体在SD大鼠妊娠期子宫中的分布情况和变化规律.[方法]选取均为正常妊娠的30只雌性SD大鼠分为妊娠3 d、妊娠5 d,妊娠7 d、妊娠1 3 d、妊娠19 d及产后第1天6组,处死各组大鼠,取子宫制作石蜡切片,经免疫组织化学SP法染色后,进行观察分析.[结果]5-HT及5-HT1A受体免疫阳性产物广泛存在于子宫内膜上皮细胞、子宫腺上皮细胞、血管内皮细胞、基质细胞、蜕膜细胞,免疫细胞及肌纤维中;5-HT在妊娠7 d和妊娠1 3 d着色最深,5-HT1A受体在妊娠13 d着色最深.5-HT及5-HT1A受体免疫阳性产物相对表达量的T检验表明,子宫内膜5-HT在妊娠7 d和妊娠1 3 d的表达较妊娠3 d、妊娠5 d和产后极显著的增加(P<0.01),较妊娠19 d显著增加(P<0.05);子宫肌层5-HT在妊娠19 d的表达较妊娠7 d显著减少(P<0.05),较妊娠1 3 d极显著的减少(P<0.01).子宫内膜5-HT1A受体在妊娠1 3 d较妊娠3 d、妊娠5 d、妊娠19 d及产后极显著的增加(P<0.01),较妊娠7 d显著增加(P<0.05);子宫肌层5-HT1A受体在妊娠13 d较妊娠各个时期极显著增加(P<0.01).5-HT和5-HT1A受体在妊娠期子宫中的表达,具有极显著的正相关性.[结论] 5-HT和5-HT1A受体在妊娠期子宫中的表达具有一定规律,5-HT通过5-HT1A受体参与妊娠的调节.%[Objective] The distribution and expression variation of 5-HT and 5-HT1A receptors in the uterus of the Sprague Dawley during pregnancy were studied. [Method] Thirty mature female SD rats with normal pregnancy were randomly divided into 6 groups: pregnancy 3 days group (3D), pregnancy 5 days group (5D), pregnancy 7 days group (7D), pregnancy13 days group (13D), pregnancy 19 days group (19D) and the first day of postpartum group (P1). The expression of 5-HT and 5-HT1AR in uterus of the rats during pregnancy cycle were studied by immunohistochemical SP method. [Result]The 5-HT and 5-HT1A R immunoreactive products mainly located in endometrial epithelial cells, the glandular epithelium, vascular endothelial cells, stromal cells, decidual cells, immune cells and muscle fiber. The strongest coloration of 5-HT was in 7D and 13D. The strongest coloration of 5-HT1A R was in 13D. The relative expression of immunoreactive product 5-HT and 5-HT1A R ofT-test showed that, compared with the 3D, 5D and P 1, the expression of 5-HT in 7D and 13D of endometrium was extremely significantly increased (P＜0.01 ), compared with the 19D, the expression of 5-HT in 7D and 13D were significantly increased (P＜0.05). Compared with the 7D, the expression of 5-HT in 19D of myometrium was significantly decreased (P＜0.05), compared with the 13D, the expression of 5-HT in 19D of myometrium was extremely significantly decreased (P＜0.01). Compared with the 3D, 5D, 7D and P0, the expression of 5-HT1A R in 13D of endometrium was extremely significantly increased (P＜0.01), Compared with the 7D, expression of 5-HT1A R in 13D of endometrium was significantly increased (P＜0.05). Compared with the 3D, 5D, 7D ,19D and P1, the expression of 5-HT1A R in 13D of myometrium was extremely significantly increased (P＜0.01). Expression of 5-HT and 5-HT1AR in pregnancy had highly significant positive correlation. [ Conclusion ] The expression of 5-HT and 5-HT1A R in the uterus in different pregnancy periods was found to adapt to some certain rules, 5-HT through 5-HT1AR are involved in the regulation of pregnancy.