大豆卵磷脂对子宫内发育迟缓仔猪肠道抗氧化和热休克蛋白70表达的影响

摘要

[目的]研究大豆卵磷脂(soya lecithine,SL)对子宫内发育迟缓(intrauterine growth retardation,IUGR)猪肠道生长、黏膜氧化应激(oxidative stress,OS)和热休克蛋白70(heat shock protein,HSP70)表达的影响.[方法]试验共选用12头7d IUGR仔猪和6头正常体重(NBW)仔猪,所有IUGR仔猪随机分成两组(n=6),分别饲喂基础人工乳(IUGR组)和添加1.5％SL的人工乳(IUGR+SL组),所有NBW仔猪饲喂基础人工乳(NBW组,n=6),试验期7d.[结果]IUGR显著降低14d仔猪空肠及其非黏膜绝对重量(P＜0.05),降低空肠黏膜总抗氧化能力(T-AOC),谷胱甘肽还原酶(GR)和谷胱甘肽过氧化物酶(GPx)活性(P＜0.05),升高MDA水平(P＜0.05),黏膜HSP70的ELISA和免疫印迹法检测结果均显著升高(P＜0.05).IUGR猪补充SL后,空肠肠段、黏膜和非黏膜绝对和相对重量均显著升高(P＜0.05)；空肠黏膜T-AOC、GPx和SOD显著升高(P＜0.05),而MDA显著下降(P＜0.05);ELISA检测结果显示HSP70蛋白水平显著降低(P＜0.05),且与SL添加水平呈显著负相关(P＜0.05).[结论]补充SL对恢复IUGR导致的仔猪肠道组织生长缓慢、HSP70表达升高和OS损伤有很好的效果.%[Objective] The effects of supplement of soya lecithine (SL) on intestinal growth, mucosal heat shock protein 70 (HSP70) expression and oxidation stress (OS) in intrauterine growth retardation (IUGR) piglets were studied. [Method] Twelve IUGR piglets and six piglets with normal birth weight were selected. All piglets were weaned on 7th d of age, and assigned equally to three groups: six NBW and six IUGR piglets were fed with control diet (IUGR), and six IUGR piglets were fed with diet supplemented with 1.5% SL (IUGR+SL) for 7 days. [ Result ] IUGR decreased (P<0.05) the absolute weigh of jejunal segment and non-mucosa. reduced (P<0.05) the mucosal capacity of total anti-oxidation (T-AOC), as well as the activity of GR and GPx, and elevated (P<0.05) the content of MDA. The mucosal HSP70 contents detected by ELISA and Western Blot were increased (P<0.05) by IUGR. After SL treatment, the absolute and relative weight of jejunum, jejunal mucosa and non-mucosa increased (P<0.05) in IUGR piglets. Supplement of SL increased (P<0.05) the mucosal T-AOC, GPx and SOD, as well as reduced (P<0.05) MDA and HSP70 content in terms of ELISA. Inverse correlations were found between SL supplementation in IUGR piglets and HSP70 contents (P<0.05). [Conclusion] SL plays an important role in recovering impaired jejunal growth, increased mucosal HSP70, and OS induced by IUGR.