鸡下丘脑高丰度miR-101的组织表达谱与功能预测分析

摘要

[Objective] The objective of this study is to comprehensively understand the chicken hypothalamus abundance miR-101 expression and potential biological function which will lay a foundation for revealing the molecular mechanisms of hypothalamic regulation of the chicken energy balance.[Method] Stem-loop quantitative RT-PCR was used to detect the miR-101 expression in 13 different tissues of Gu-shi chicken at four developmental stages.The algorithms PicTar was used to predict miR-101 putative target genes.Gene Ontology,pathway and stratified enrichment were analyzed respectively to target genes.[Result]MiR-101 expression exhibitted striking temporal and tissue specificities.The miR-101 expression levels in different tissues of embryonic stages were significantly higher than the expression levels in homologous tissues of hatched chicks,the expression levels in the brain tissues were also significantly higher than the other tissues examined.MiR-101 had abundance enrichment in hypothalamus at 14 d embryonic age and in small intestine and crura muscle at 17 d embryonic age.Predicted target genes of miR-101 were significantly enriched in biological regulation,metabolic processes,developmental processes and cellular processes.Bioinformatics analysis of the nervous system development displayed that the regulatory function of miR-101 was mainly involved in brain development,neuron differentiation,regulation of neurogenesis,glial cell differentiation,astrocyte differentiation,and so on.[Conclusion] MiR-101 was the high expression miRNA in brain tissue,and may play an important role in regulating biological processes related to brain development.%[目的]全面了解鸡下丘脑高丰度miR-101的组织表达情况和潜在生物学功能,为揭示下丘脑调控鸡机体能量平衡的分子机制奠定基础.[方法]采用茎-环定量RT-PCR方法检测miR-101在固始鸡4个发育阶段、1 3种组织中的表达,利用Pictar算法预测miR-101的靶基因并对预测靶基因分别进行Gene Ontology分析、通路分析和分层富集.[结果]miR-101的表达具有明显的时序特征和组织特异性,胚胎期各组织中的表达水平明显高于出壳后,脑部各组织中的表达水平明显高于其它被检测的组织,并在14胚龄下丘脑以及17胚龄小肠和腿肌中高丰度富集；miR-101的预测靶基因在生物学调节、代谢过程、发育过程和细胞过程等基本生物学过程中显著富集；针对神经系统发育的生物信息学分析显示,miR-101调控功能主要涉及脑发育、神经元分化、神经发生调节、神经胶质细胞分化和星形胶质细胞分化等生物学过程.[结论]miR-101为脑部组织高丰度表达miRNA,在脑发育相关的许多生物学过程中可能发挥重要的调节作用.