环介导等温扩增技术在食品安全检测领域的应用研究进展

摘要

环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP)是近年发展起来的一种新型核酸检测技术其采用特异识别靶序列上6个位点的4条引物和一种具有链置换活性的DNA聚合酶,在等温条件下进行核酸扩增,与传统核酸检测技术(PCR法、实时荧光定量PCR法)相比,具有操作简单、特异性强、灵敏度高、可肉眼判读结果等优点核酸检测是食品安全检测技术的一个重要手段,本文综述了LAMP技术在食品微生物检测、转基因成分检测、过敏原成分检测和动物源性成分检测领域的应用研究进展,探讨了LAMP技术在食品检测领域的发展前景,以期为食品快速、高通量检测技术建立提供参考%loop-mediated isothermal amplification(LAMP) is a novel nucleic acid amplification method developed in recent years.The nucleic acid amplification is performed ender isothermal conditions by a set of four specially designed primers and a DNA polymerase with strand displacement activity.Compared with the traditional nucleic acid detection technology (PCR and real-time fluorescence quantitative PCR),it has the advantages of easy operation,high specificity,sensitivity and visual interpretation.Nucleic acid detectiou is an important means of food safety detection.This review summarized the application research progresses mainly focused on food safety detection,including food microorganisms testing,genetically modified organisms testing,food allergens testing and food derived ingredients testing.In addition,the perspectives of LAMP in food detectiou were discussed for better and high volume testing in food safety detection.