响应面法优化石上柏穗花杉双黄酮提取工艺

摘要

采用响应面法优化石上柏穗花杉双黄酮微波提取工艺条件.研究了微波功率、提取时间、固液比和提取温度等对石上柏中穗花杉双黄酮得率的影响,并采用响应面实验设计和多元二次回归分析,优化了此提取工艺.通过实验得出穗花杉双黄酮最佳提取工艺条件:固液比为1∶15 g/mL,微波功率为400W,提取时间为37 min,提取温度为47℃.在此条件下,穗花杉双黄酮的得率达到了0.33％±0.08％.与乙醇回流提取法和索氏提取法相比,该方法节省时间、节约能量、提取效率高、控制方便.可用于石上柏穗花杉双黄酮的提取.%In this study,microwave assisted extraction (MAE)was firstly employed to extract amentoflavone from Selaginella doederleinii by response surface method.The effect of factors on the yield of amentoflavone was investigated,including microwave power,extraction time,solid-liquid ratio and extraction temperature.The response surface experiment design and multiple quadratic regression analysis were ordered to optimize the extraction technology.The results showed that the best extraction conditions were as follows:solid-liquid ratio 1 ∶ 15 g/mL,microwave power 400 W,temperature 47 ℃,microwave extraction time 37 min.Under this condition,amentoflavone yield reached 0.33％ ± 0.08％.Compared with ethanol reflux extraction and soxhlet extraction,MAE was not only increased the yield of target,but also reduced extraction time.The method is simple,accurate and efficient for the extraction of amentoflavone from Selaginella doederleinii.