Objective:To choice better edible rocket cultivar and germination time by investigating the effect of germination on the growth status and glucosinolates metabolism.Methods:Two cultivated varieties of rockets "Banye" and "Dongsheng" were investigated about the related indexes of length,weight,water content,free amino acid,soluble sugar and glucosinolates metabolism of the 0,2,4,6 and 8th day during germination.Results:The sprout length,fresh weight and water content of two cultivars gradually increased.In "Banye" and "Dongsheng",free amino acid content reached the peak value at day 8 with 7.68 μg/sprout and 7.64 μg/sprout,respectively.The soluble sugar content got the highest value at day 6 with 216.8 μg/sprout and 203.5 μg/sprout,respectively.And the total glucosinolates content in "Banye" and "Dongsheng" both reached the highest level at day 2 but had minimal content at day 8 with 53.74 nmol/sprout and 55.87 nmol/sprout,respectively.In addition,the isothiocyanates formation both increased to the maximum at day 4 in "Banye" and "Dongsheng",with 66.82 nmol/sprout and 89.76 nmol/sprout,respectively.Myrosinase activity reached to the highest at day 2 and day 4,respectively,but there was no significant difference between the two cultivars at day 8.Conclusions:The quality of seeds can be improved by germinating,in the period of germination,the degradation of carbohydrate was earlier than that of the protein.Glucosinolates were degraded during the germination.Temporal and spatial sequence existed in myrosinase activation and formation of isothiocyanates.In general,the 4-day "Dongsheng" sprouts were more suitable for daily diet.%目的:研究发芽过程中芝麻菜生长状况和硫苷代谢的变化情况,从而选出适宜食用的芝麻菜品种及其生长期.方法:以“板叶”和“东升”两个品种的芝麻菜种子为试材,发芽0、2、4、6和8d后测定其芽长、株重、含水量、游离氨基酸含量、可溶性糖含量及硫苷代谢相关指标.结果:随着发芽时间的延长,两个品种芝麻菜芽苗的芽长、株重和含水率逐渐升高;“板叶”和“东升”中游离氨基酸含量均在第8d最高,分别为7.68μg/株和7.64 μg/株;可溶性糖含量均于第6d达到最高,分别为216.8μg/株和203.5μg/株.总硫苷含量在发芽2d达到最高,而发芽8d后降至最低,分别为53.74 nmol/株和55.87 nmol/株;异硫氰酸盐形成量均在发芽4d最高,分别达到66.82 nmol/株和89.76 nmol/株,而黑芥子酶则分别在第2d和第4d活性最高,发芽8d后两者无显著差异.结论:发芽可以提高种子的品质,芽苗生长过程中,储藏性糖类物质的降解先于蛋白质,硫苷被不断降解,黑芥子酶活性和异硫氰酸盐的形成存在时空差异.综合来看,“东升”的植株大小、所合营养物质,GLs及ITCs含量较“板叶”更高,芽苗中主要关注的功能成分为ITCs,选用4d为较合适的发芽时间.
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