荧光分光光度法测定水样中氧氟沙星的含量

摘要

The fluorescence characteristics of ofloxacin and its concentration in water samples were investigated by fluorescence spectrophotometry. The results illustrate that ofloxacin solution with pH value of 7 has the strongest fluorescence intensity at excitation wavelength 293 nm and emission wavelength 471 nm. The fluorescence intensity of ofloxacin decreases with the temperature increases from 20 ℃ to 35 ℃. When the substances in the water sam-ples do not interfere with the fluorescence characteristics of ofloxacin, the concentration of ofloxacin can be deter-mined at excitation wavelength 293 nm and emission wavelength 471 nm by a fluorospectro photometer. The fluores-cence intensity of ofloxacin solution with pH value of 7 is proportional to its concentration with the range of 0. 05~1. 0 mg/L at the temperature of 20 ℃. The detection limit is 0. 43 μg/L, the relative standard deviation is less than 1. 6 % ( n=6 ) , and the recovery range is 97. 5 % ~100. 9 %. The method is suggested to be used to deter-mine the ofloxacin in batch water samples.%采用荧光分光光度计分析了氧氟沙星的荧光特征及其在水样中含量。研究发现,在激发波长293 nm和荧光发射波长471 nm处pH=7的氧氟沙星水溶液具有最大荧光强度；在20~35℃范围内,氧氟沙星水溶液的荧光强度随温度升高而降低。当水样中无干扰氧氟沙星荧光特征的物质时,可在20℃、pH=7条件下,于激发波长293 nm和荧光发射波长471 nm处测定水样中氧氟沙星的含量,氧氟沙星的荧光强度与其浓度正相关,相关系数为0．9990,线性范围为0．05~1．0 mg/L,检出限为0．43μg/L,相对标准偏差小于1．6%(n=6),加标回收率为97．5%~100．9%,该方法适用于快速测定水样中氧氟沙星的含量。