Flow Injection Spectrophotometric Determination of the Antibacterial Levofloxacin in Tablets and Human Urine

摘要

A sensitive and fast flow-injection(FI)spectrophotometric method for the determination of levofloxacin based on the formation of a colored product upon oxidation with N-bromosuccinimide(NBS)in acidic medium is proposed.Optimiz-ation of chemical and FI variables has been made.Under the optimized conditions,the sampling rate was over 90h~(-1),the calibration curve obtained was linear over the range 10-300 mu g centre dot mL~(-1),and the detection limit was 3 mu g centre dot mL~(_1).The proposed method was successfully applied to the determination of levofloxacin in pharmaceuti-cals and human urine samples.It was also found that the excipients in the commercial tablet preparation did not interfere with the assay.Results are precise(RSD < 2.7%;n = 10)and in agreement with those found by the reference high pressure liquid chrom-atography(HPLC)procedure.