To study the effects of tea polyphenols(TP) on the development and progression of colitis-associated colorectal cancer (CAC) in mice with long-range high-fat diet. Five-week-old BALB/c mice were randomized 5 different groups: HFD alone,AOM/DSS alone, HFD + AOM/DSS, HFD + AOM/DSS + TP and the untreated group. CAC was induced by intraperitoneal injection of cyclic azoxymethane(AOM) and drinking 2% dextran so-dium sulfate (DSS). Quantitative real-time PCR was utilized for measuring the expression levels of TNF-α,IL-6, COX-2,IL-4,IL-10 and C-myc. Western Blotting was adopted for detecting the protein expression levels of β-cate-nin and P-GSK3β. By HE staining to observe colorectal morphological structure,immune cell infiltration. Results show that TP decreased the expression level of TNF-α, IL-6, COX-2, β-catenin and C-myc (P<0.05) and in-creased the expression level of IL-4,IL-10 (P<0.05) compared to the HFD+AOM/DSS. And TP reduced for-mation of colorectal cancer in the AOM/DSS induced CAC mice model, inhibited colorectal shorten, maintained the integrity of intestinal barrier. The study dates suggest that TP can inhibiting the activation of Wnt signaling pathway,and has a obvious inhibitory effect on CAC.%探讨茶多酚(TP)对长期高脂饮食小鼠结肠炎相关性结肠癌(CAC)发生发展的影响及其可能机制.将5周龄BALB/c小鼠50只随机分为对照组、HFD组、AOM/DSS组、HFD+AOM/DSS组和HFD+AOM/DSS+TP组,采用三次循环腹腔注射氧化偶氮甲烷(AOM)以及饮用2%葡聚糖硫酸钠(DSS)建立CAC模型.通过实时荧光定量PCR检测TNF-α、IL-6、COX-2、IL-4、IL-10、β-catenin和C-myc 的mRNA表达水平;通过Western Blotting 检测β-catenin、P-GSK3β和GSK3β蛋白表达量;通过HE染色观察结直肠形态结构,免疫细胞浸润程度.结果表明:与HFD+AOM/DSS组相比茶多酚上调了TNF-α、IL-6、COX-2、β-catenin和C-myc的表达(P<0.05),下调了IL-4、IL-10的表达(P<0.05),促进了β-catenin的降解,显著降低AOM/DSS诱导CAC小鼠模型结直肠肿瘤的发生、抑制了结直肠缩短、维护了肠道屏障的完整.说明茶多酚能缓解炎症反应并抑制了Wnt信号系统的活化,对CAC有明显抑制作用.
展开▼
