不同产地三七皂苷的HPLC指纹图谱与含量的比较研究

摘要

目的 通过栽培药材的皂苷HPLC分析,讨论异地种植三七的可行性.方法 根据《中国药典》2010年版一部,采用HPLC检测云南省不同产地三七地下部分主要皂苷的含量,建立模式指纹图谱,并进行比较.结果 产于云南红河泸西与弥勒的三七,其剪口、主根和大根的皂苷指纹图谱相似度均大于0.99,含量均高于文山产三七.结论 虽然三七的栽培条件苛刻,但只要选择适宜的环境,加强管理,三七的异地种植是可行的.%Objective Through the HPLC analysis of cultivated crude herbal materials to discuss the feasibility of strange land plantation of Panax notoginseng. Methods According to the methods of "Chinese Pharmacopoeia"(2010) established HPLC fingerprint mode and determined the content of five major saponins. Results To compare the HPLC fingerprint of cultivated notoginseng, the similarity of the strange land plantation (Mi-Le and Lu-Xi) and homeland plantation (Wen-Shang) are greater than 0.99. The five major saponin contents of different parts (rhizome, main roots and lateral roots) in strange land are relative higher than homeland. Conclusion It is suggested that even though the cultivation of notoginseng is difficult, the strange land cultivation is feasibility while choose the appropriate environment and make good management.