Objective To establish a method for the determination of menthol in anti-inflammatory antipruritic spiritus by GC. Methods GC was performed on a CP-Sil 5 CB column (25m × 0.32mm × 0.4um) using FID as the detector. Inlet temperature was set at 250 ℃ and column temperature was 280 ℃ (temperature program: initial temperature 60 ℃, maintained lmin, then rise to 280 ℃ with the rate of 12 ℃ / min, maintained 3min); detector temperature was 300 ℃ ; split injection , split ratio 20:1; injection volume 1μL. Results The standard curve was linear in the range of 50.8 ~ 609.6mg / L, r = 0.999 69, the average recovery was 99.10% with RSD of 0.87% (n = 6). Conclusion The method was simple, fast, accurate, reproducible and could be used for the determination of menthol in anti-inflammatory antipruritic spiritus.%目的 建立GC法测定消炎止痒醑中薄荷脑的含量.方法 采用CP-Sil 5 CB色谱柱(25m×0.32mm×0.4μm),FID检测器.进样口温度250℃;柱温280℃(程序升温:初始温度60℃,保持1min,再以12℃/min的速度升至280℃,保持3min);检测器温度300℃;分流进样,分流比20:1;进样量1μL.结果 薄荷脑进样量在50.8 ～ 609.6mg/L范围内与峰面积呈良好线性关系,r=0.999 69,平均回收率为99.10％,RSD为0.87％(n=6).结论 本测定方法简便、快速、准确、重复性好,可作为消炎止痒醑的含量测定方法.
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