拟草果乙酸乙酯部位抗炎作用及机制研究

摘要

Objective To study the anti-inflammatory activity of ethyl acetate extracts from Amomum paratsao-ko and its mechanism.Methods The increasing in celiac vascular permeability caused by acetic acid,rat carboxymethyl cellulose (CMC) model,the weight of cotton granuloma in mice and carrageenan-induced hind paw edema model were employed to evaluate the anti-inflammatory activity of ethyl acetate extracts from Amomum paratsao-ko.Ultraviolet spectrophotometry and enzyme-linked immunosorbent assay were used for the detection of prostaglandin E2 (PGE2),tumor necrosis factor α (TNF-α),interleukin-1 β (IL-1β),nitric oxide (NO) and malondialdehyde (MDA).Result The ethyl acetate extracts from Amomum paratsao-ko significantly decreased the capillary hyperpermeabitity in mice,inhibited the migration of white blood cell in CMC capsule,reduced granuloma formation in mice,relieved the carrageenan-induced hind paw edema,decreased the secretion of inflammatory mediators PGE2,IL-1β and TNF-α,and reduced the accumulation of NO and MDA in vivo.Conclusion The ethyl acetate extracts from Amomum paratsao-ko had obvious anti-inflammatory activity,which may be related to the inhibition on inflammatory factors and the reduction of peroxidation products.%目的 探讨拟草果乙酸乙酯部位的抗炎作用并初步研究其作用机制.方法 采用小鼠醋酸致毛细血管通透性模型、大鼠羧甲基纤维素(CMC)囊模型、大鼠棉球肉芽肿模型、大鼠角叉菜胶足肿模型来评价拟草果乙酸乙酯部位的抗炎作用,并采用紫外分光光度法和酶联免疫吸附法检测炎性组织中前列腺素E2(PGE2)、肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)、一氧化氮(NO)和丙二醛(MDA)的含量.结果 拟草果乙酸乙酯部位可以明显降低醋酸致小鼠毛细血管通透性、抑制大鼠CMC囊白细胞游走及棉球肉芽组织增生,减轻大鼠角叉菜胶足肿胀,减少炎性介质PGE2、IL-1β及TNF-α的分泌,降低大鼠体内NO和MDA的堆积.结论 拟草果乙酸乙酯部位可能通过抑制炎症因子的释放以及减少过氧化产物来发挥其抗炎作用.