子痫前期患者胎盘组织中降钙素受体刺激肽-3(CRSP-3)的表达及意义

摘要

目的:研究子痫前期患者胎盘组织中降钙素受体刺激肽-3(CRSP-3)的表达与正常妊娠者之间的差异及其临床意义.方法:采用免疫组织化学方法检测住院分娩的50例子痫前期患者(子痫前期组:轻度子痫前期25例,重度子痫前期25例)和30例正常晚期妊娠孕妇(对照组)胎盘组织中CRSP-3的表达定位,Real-time PCR及Western blotting方法检测胎盘组织中CRSP-3mRNA和蛋白的表达.结果:子痫前期和正常妊娠者胎盘组织细胞滋养细胞和合体滋养细胞中均表达CRSP-3.子痫前期患者胎盘组织中CRSP-3mRNA及其蛋白表达水平均高于正常妊娠组(P均＜0.05).其中重度子痫前期患者胎盘组织中CRSP-3mRNA和蛋白表达水平显著高于正常对照组(P均＜0.01),虽重度子痫前期胎盘组织中CRSP-3表达略高于轻度子痫前期,但组间无统计学差异(P均＞0.05).结论:CRSP-3上调可能参与子痫前期的发病,但是与疾病的进展及严重程度无关.%Objective:To explore calcitonin receptor stimulating peptide-3 (CRSP-3) in the placenta relationship with the pathology of preeclampsia.Methods:Fifty cases of preeclampsia (25 cases of mild and severe preeclampsia each) were enrolled in the study and 30 cases of normal pregnant women were selected as the control.The expression location of CRSP-3 was detected by immunohistochemistry,and the expression level of CRSP-3 in placental tissues was detected by RT-qPCR and Western blotting.Results:CRSP-3 protein was distributed in the cytotrophoblast cells and syncytiotrophoblast cells.The expressions of CRSP-3mRNA and protein in the preeclampsia group were significantly higher than those in the control (P＜0.05).Especially,CRSP-3mRNA and protein levels in severe preeclamptic women were higher than those in the control (P＜0.01).CRSP-3mRNA and protein levels in severe preeclamptic women were higher than those in mild preeclamptic women,but without any significance (P＞0.05).Conclusion:The up-regulation of CRSP-3 in placenta ofpreeclamptic patients may be associated with the etiology of preeclampsia,but may have no relation with progress and degree of preeclampsia.