SEPT11基因在小鼠睾丸精子发生过程中的表达特征

摘要

Objective To investigate the expression characteristic of SEPT11 in mouse testis and its role in spermatogenesis.Methods Real-time PCR,Western blotting and immunofluorescence were applied to study the expression characteristics of gene SEPT11 in mouse testis at different postnatal week and from Sertoli cell-selected Ar knockout mice (SCARKO) and Ar knockout (ARKO) adult mice.Results The expression of SEPT1l was highest during postnatal 2 weeks,and then fall down.SEPTIN11 colocalized with mature sperm attached with Sertoli cells in the testis from mice at postnatal 6 weeks.The expression of SEPTIN11 in SCARKO and ARKO mice testis were much lower compared with the wild type (P＜0.01),the mRNA level was significantly higher (P＜0.01).SEPTIN11 localized to the tail of mature sperm collected from epididymis.Conclusion The new born mice had the highest expression level of SEPT11.SEPT1N11 localized to the tail of mature sperm.Knock-down of Ar increased the expression of SEPT11.%目的 初步探讨Septin 11 (SEPT11)基因在精子发生中的作用.方法 通过实时定量PCR、蛋白免疫印迹及免疫荧光等方法检测SEPT11在不同周龄野生型小鼠以及成年睾丸支持细胞激素受体(Ar)特异性敲除小鼠(SCARKO)和Ar全敲除(ARKO)小鼠睾丸中的表达特征.结果 SEPT11基因小鼠出生2周内高表达,随后表达水平降低,出生6周后出现并与未释放的成熟精子共定位且高表达.与野生型小鼠相比,SEPTIN 11在SCARKO小鼠和ARKO小鼠睾丸中表达降低(P＜0.01),mRNA水平显著升高(P＜0.01);SEPTIN11在成熟精子中定位于尾部.结论 SEPT11基因在小鼠出生时表达最高;SEPTIN11在小鼠成熟精子中定位于尾部;Ar的敲减提高了SEPT11的表达.