该研究立足于河南天冠企业集团纤维素乙醇项目,以酶解糖化工艺对木聚糖酶的需求为出发点,利用黑曲霉X06作为产酶菌株,采用固体发酵工艺,通过正交设计试验,优化了培养基配方和发酵控制工艺,最优方案:麸皮:玉米芯=6:4、硝酸铵4%、尿素1%、磷酸二氢钾0.4%、硫酸镁0.2%、初始含水量65%、初始pH=4.0、温度28℃、环境相对湿度70%、72 h酶活达到10096.74 IU/g.这一方案在生产中得到进一步放大和优化,所生产的固体木聚糖酶应用在秸秆酶解工艺中,酶解液中木糖含量提高了66.9%.%Based on the needs of xylanase in the cellulosic ethanol project of Henan Tianguan Group Co., Ltd., Using Aspergillus niger X06 as xylanase-producing strain, through orthogonal experiment, the optimal solution of solid state fermentation was obtained as follows: the wheat bran to corn cob ratio is 6:4,the NH4NO3 content is 4％, the CO(NH2)2 content is 1％, the KH2PO4 content is 0.4％, the MgSO4·TH2O content is 0.2％, the initial moisture content is 65％, the initial pH value is 4.0, the incubation temperature is 28 ℃, the relative humidity of the environment is 70％. The activity of xylanase could reach 10 096.74 IU/g in 72 h. This program has been amplified and further optimized in large-scale production. The production of solid xylanase has been used in straw hydrolysis process, and the xylose content increased by 66.9％.
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