比较盐酸法舒地尔对不同离体动物眼房水流畅系数的影响

摘要

Objective To evaluate the influence of a selective Rho-associated protein kinase inhibitor (fasudil hydrochloride) on outflow facility in enucleated porcine,rabbit and bovine eyes.Methods At the constant perfusion pressure of 15 mm-Hg (1 kPa =7.5 mmHg),the baseline coefficient of outflow facility (C0) of the isolated porcine,rabbit and bovine eyes was recorded respectively.The enucleated porcine eyes were divided into two groups randomly (n =6),and they were control group and experimental group.The same grouping method was also used-C0 the ribbit and bovine eyes.The control group was subjected to GPBS perfusion,while the experimental group was treated with 100 μmol · L-1 fasudil solution,followed by recording the experimental coefficient of outflow facility (C1),as well as calculating ΔC (ΔC =C1-C0) and ΔC％ (ΔC％ =ΔC/C0).Finally,the paired t test and one-way analysis of variance were performed using SPSS 17.0.Results As for porcine eyes,the ΔC％ of the control group was (17.83 ± 3.84) ％ while the experimental group was (44.00 ± 6.44) ％;as for rabbit eyes,the ΔC％ of the control group was (15.50 ± 2.93) ％,while the experimental group was (31.67 ±6.54)％;as for bovine eyes,the ΔC％ of the control group was (11.67 ± 1.17)％,while the experimental group was (37.17 ± 4.48)％.The ΔC％ in the experimental group was significantly increased when compared with the control group in three animals,with significant difference (all P ＜ 0.05).There was no statistical difference in ΔC％ of three experimental groups among different kinds of animals (all P ＜ 0.05).Conclusion Fasudil can improve outflow facility in enucleated eyes of animals,and it can redistribute aqueous humor drainage to a wider area through directly regulating the cytoskeleton of cells and matrix,resulting in increased coefficient of outflow facility.%目的 评价选择性Rho激酶抑制剂盐酸法舒地尔对离体猪眼、兔眼及牛眼的房水流畅系数的影响.方法 在15 mm-Hg(1 kPa=7.5 mmHg)恒压灌注条件下,先记录离体猪眼、兔眼及牛眼的基线房水流畅系数(C0),然后随机将眼球分为对照组及实验组,每组6只,对照组使用GPBS、实验组使用100 μmol·L-1盐酸法舒地尔溶液继续灌注,记录实验房水流畅系数(C1),并计算房水流畅系数增加值ΔC(ΔC=C1-C0)及房水流畅系数增加百分比ΔC％(ΔC％=ΔC/C0),利用SPSS 17.0进行配对t检验及单因素方差分析.结果 猪眼对照组、实验组的ΔC％分别为(17.83±3.84)％、(44.00±6.44)％;牛眼对照组、实验组的ΔC％分别为(11.67±1.17)％、(37.17±4.84)％;兔眼对照组、实验组的ΔC％分别为(15.50±2.93)％、(31.67±6.54)％,三种动物眼的实验组ΔC％较对照组均明显增加,差异均有统计学意义(均为P＜0.05).三种不同动物离体眼实验组的ΔC％之间差异无统计学意义(F=1.064,P=0.370).结论 盐酸法舒地尔可以增加不同种类离体动物眼房水流畅系数,其作用可能是通过调节小梁房水流出道的细胞及基质骨架结构,重新分布房水引流到一个更广泛的区域而发挥增加房水流畅系数的作用.