高脂血症大鼠模型几种造模方法的筛选及优化

摘要

To filter an ideal method which can build hyperlipidemia rat model out of different modeling methods,we divided 40 rats stochastically as the blank group,the model Group Ⅰ,the model Group Ⅱ, and the model Group Ⅲ,and there were 10 rats in each group.Except the blank group,three different modeling methods were used to build Hyperlipidemia rat models in the other three model groups.GroupⅠ:high fat diet;Group Ⅱ:normal diet respectively with intragastric administration of fat emulsion;model Group Ⅲ:before intragastric administration of fat emulsion the same as model group Ⅱ,give an intraper-itoneal injection of vitamin D3 60 000 U/kg.We took the orbital venous plexus blood of all the rats both at the end of 1,3 and 5 weeks during the models,then detected the seral levels of TC,TG,HDL-C ,LDL-C, SOD,MDA,NO and MPO,and detected the levels of body weight and the liver index of the rats in each group.The results showed that the rats Group Ⅱ and model Group Ⅲ became hyperlipoidemic and the model of hyperlipidemia was formed successfully.Compared with model Group Ⅰ and Group Ⅱ,the model-ing cycle of model group Ⅲ was significantly reduced,which saved the cost of the experiment,and was more stable.This study revealed that Group Ⅲ it is an ideal method of building hyperlipidemia rat model.%通过几种具有代表性的造模方法诱导高脂血症大鼠模型，观察实验性高脂血症模型大鼠血清SOD 活性、MPO、NO 和 MDA 含量水平，筛选出理想的高脂血症造模方法。将健康 SPF 级雄性 SD 大鼠40只随机分为空白组、模型组Ⅰ～Ⅲ，每组10只。空白组基础饲料喂养；各模型组分别采用高脂饲料喂养和高脂乳剂灌胃法诱导造模，模型组Ⅰ：高脂饲料配方喂养；模型组Ⅱ：基础饲料喂养配合高脂乳剂灌胃；模型组Ⅲ：一次性腹腔注射维生素 D3600000 U/kg 后，饲养方法同模型组Ⅱ。在造模1周、3周和5周于大鼠眼眶静脉丛采血，提取血清，全自动生化仪测定大鼠血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白-胆固醇（LDL-C）、高密度脂蛋白-胆固醇（HDL-C）、超氧化物歧化酶（SOD）活性、髓过氧化物酶（MPO）、一氧化氮（NO）、丙二醛（MDA）含量水平并计算肝指数。模型组Ⅱ和模型组Ⅲ均出现血脂代谢紊乱，NO、SOD、MDA 和肝指数水平差异显著（P ＜0．05），提示高脂血症模型构建成功；模型组Ⅲ造模周期明显较模型组Ⅱ缩短；模型组Ⅰ中 HDL-C、MPO、NO、SOD 和 MDA 水平差异不显著（P ＞0．05）。模型组Ⅲ的造模周期明显较模型组Ⅰ、模型组Ⅱ缩短，且效果明显，是建立高脂血症大鼠模型的理想方法。