传染性法氏囊病病毒 RT-PCR 检测及 VP2基因序列分析

摘要

The foundation was laid to carry out diagnostic reagents and genetically engineered vaccine re-search of IBDV.IBDV liaocheng isolate was isolated from suspected IBD chickens from a chicken farm in Liaocheng city.Viral dsRNA was extracted from the isolate,VP2 gene was acquired by RT-PCR.The ho-mology of the sequencing results was between 79.2% and 98.7% campared with sequences of IBDV VP2 reported from GenBank.Phylogenetic tree analysis between the VP2 gene and 22 sequenced VP2 gene re-ported from GenBank,confirmed the closest homology with SDH1 strain.%对传染性法氏囊病毒聊城分离株 VP2基因序列分析，为进一步开展 IBDV 诊断试剂及基因工程疫苗研究奠定基础。从山东聊城地区某养鸡场疑似鸡传染性法氏囊病病鸡初步分离到一株记为聊城分离株的 IBDV 毒株，从该分离株中提取总 RNA，采用 RT-PCR 技术扩增 VP2基因。测序结果与 GenBank 中报道的 IBDV VP2序列同源性在79．2％～98．7％。将 VP2基因序列与 GenBanK 上报道的22个序列进行系统发育进化树分析，证实 VP2基因与 SDH1株同源性最近。