目的:研究左归丸对小鼠未成熟卵母细胞体外核成熟的影响.方法:制备左归丸含药血清,将生发泡(germinal vesicle,GV)期卵母细胞分别在不同采血时间获取的左归丸含药血清培养液中进行体外培养,观察左归丸含药血清对生发泡破裂(germinal vesicle breakdown,GVBD)和第一极体(the first polar body,PB1)排出的时效关系.结果:药物血清组卵母细胞GVBD的发生率高于正常血清组和对照组,于培养后4h差异最显著(P＜0.01);药物血清组卵母细胞PB1的发生率高于正常血清组和对照组,于培养后18h差异最显著(P＜0.01).结论:2～2.5h左归丸含药血清对未成熟卵母细胞体外核成熟具有明显促进作用.%Objective: To investigate the effect of Zuoguiwan in vitro on nuclear maturation of immature mouse oocytes.Methods: The serum containing Zuoguiwan were prepared. The immature oocytes were isolated from the ovaries of the mouse. The GV oocytes were cultured in the culture media supplemented with Zuoguiwan serum with different blood sampling time, respectively. The rates of GVBD and PB1 extrusion were detected. Results: There was higer GVBD in several Zuoguiwan serum groups than that in the controls and normal serum group. And the difference was the most notable at 4th hours. There was higer PB1 in Several Zuoguiwan serum groups than that in the controls and normal serum group. And the difference was the most notable at 18th hours. Conclusion: 2～2.5h Zuoguiwan serum improved in vitro nuclear maturation of immature mouse oocytes significantly.
展开▼
