DAPK甲基化及HPV感染与维吾尔族妇女宫颈病变的相关性研究

摘要

Objective:To investigate the correlation of death-associated protein kinase (DAPK) methylation and HPV infection with cervical lesions in Xinjiang Uighur,and provide references for the diagnosis and treatment of cervical lesions.Methods:30 cases ofchronic cervicitis,30 cases of CIN Ⅰ,30 cases of CIN Ⅱ/Ⅲ and 30 cases of cervical squamous cell carcinoma were selected,the methylation levels of DAPK were detected by MSP method and HPV infecion were determined by PCR,the relationship between which were analyzed.Results:(l)The methylation rate ofDAPK gene in chronic cervicitis,cervical intraepithelial neoplasia Ⅰ (CIN Ⅰ),CIN Ⅱ /Ⅲ and invasive squamous carcinoma of cervix were respectively 3.33％,10％,36.7％,63.3％.The methylation rate of DAPK in the SCC group was significantly higher than those in the other three groups (P＜0.05).(2)The positive rate of HPV infection in four groups were respectively 43.3％,56.7％,6.7％,93.3％,which was significantly higher in the SCC group than those in the other three groups(P＜0.05).(3)The level of methylation ofDAPK gene in cervical lesions and positive rate of HPV-DNA was positively correlated.Conclusion:The methylation of DAPK gene promoter and HPV infection played an important role in the development of cervical lesions in Xinjiang Uighur women,especially squamous cell carcinoma of the cervix.The methylation of DAPK gene promoter and HPV infection were positively correlated,combined detection of which may contribute to the diagnosis of squamous cell carcinoma of the cervix in Xinjiang Uighur women.%目的:探讨死亡相关蛋白激酶(death associated protein kinase,DAPK)甲基化及人乳头瘤病毒(humanpapillomavirus,HPV)感染与新疆维吾尔族妇女宫颈病变的相关性,为维吾尔族妇女宫颈病变的诊断和治疗提供参考依据.方法:选择新疆维吾尔自治区人民医院2008年至2009年收治的宫颈非癌组织30例、宫颈上皮内瘤变(CIN)Ⅰ 30例、CIN Ⅱ/Ⅲ30例、宫颈鳞癌组织30例患者的组织标本为研究对象,运用MSP法检测宫颈组织中DAPK基因启动子甲基化水平及PCR检测各组织中HPV的感染情况,对二者进行相关性分析.结果:(1)宫颈非癌组织、CINⅠ、CIN Ⅱ/Ⅲ、宫颈鳞癌组织中DAPK基因甲基化的检出率分别为3.33％、10％、36.7％、63.3％,宫颈鳞癌组的甲基化率明显高于其它三组,差异有统计学意义(P＜0.05);(2)四组HPV的阳性率分别为43.3％、56.7％、76.7％、93.3％,宫颈鳞癌组的 HPV感染率明显高于其它三组,差异有统计学意义(P＜0.05),HPV的感染率和宫颈病变程度成正相关(P＜0.05).(3)宫颈病变组织中DAPK基因的甲基化水平与HPV-DNA阳性率呈正相关.结论:DAPK基因启动子的甲基化及HPV感染在新疆维吾尔族妇女宫颈病变尤其是宫颈鳞癌的发生过程中可能起重要作用,二者呈正相关,联合检测二者可能有助于新疆维吾尔族妇女宫颈鳞癌的诊断.