脊髓薄片器官型培养技术的研究进展

摘要

The organotypic spinal cord slice is a technology which culture and study spinal cord slice by organotypic culture invitro,living slicing machine and microporous membrane.The characteristics of the technology is simple operation,visual observation,long time experiment in vitro and facilitating applied experimental factors.The method provide more technical support and new ways for studying on pathology and physiology of spinal cord in vitro,but the application and report of the technology in domestic is few.Because its high application value,this paper makes a review on development,characteristic,announcement,and application of organotypic spinal cord slice.%脊髓薄片器官型培养技术是一项借助体外器官培养技术,通过活体切片机、微孔膜技术的应用,将脊髓一部分分离出来进行培养、研究的技术,该技术具有操作简单,观察直观,且可长时间进行体外实验,便于施加实验因素等特点,为体外研究脊髓的生理、病理变化提供了更多的技术支持和新的途径,但是该项技术在国内目前应用、报道很少,而其应用价值极高,故本文就脊髓薄片器官型培养技术的发展、特点、方法、注意事项、应用等方面对该项技术做一综述.