不同植入材料对表皮葡萄球菌和结核杆菌粘附和生物膜形成的影响

摘要

目的:探讨表皮葡萄球菌和结核杆菌在不同生物材料表面粘附性和生物膜形成能力的差异,为临床骨科材料的选择提供理论参考.方法:采用常规方法进行细菌分离和菌种鉴定.粘附能力检测采用菌落计数法,生物膜形成能力检测采用96孔板结晶紫染色法.结果:结核杆菌和表皮葡萄球菌在骨组织均有最强的粘附和生物膜形成能力,表皮葡萄球菌在三种材料上的粘附和生物膜形成能力均高于结核杆菌,表皮葡萄球菌和结核杆菌在铁合金和不锈钢粗糙表面上的粘附和生物膜形成能力均显著高于其光滑表面(P＜0.05).结论:表皮葡萄球菌和结核杆菌在不同生物材料上具有不同的粘附和生物膜形成能力,不同种属细菌在不同材料的粘附和生物膜形成能力为临床骨科生物材料的选择提供了理论依据.%Objective:To investigate the differences in adherence and biofilm formation between Staphylococcus epidermidis and Mycobacterium tuberculosis isolates on different biomaterial implant surfaces and provide a theoretical reference for the selection of clinical orthopedic materials.Methods:Bacteria were isolated and identified by conventional methods.The ability of adherence was detected by colony counting method and the biofilm formation ability was measured by 96-well crystal violet staining method.Results:S.epidermidis and M.tuberculosis isolates had the strongest adherence and biofilm formation ability on bone material.S.epidermidis isolates had a higher adherence and biofilm formation on the three materials than that of M.tuberculosis.In addition,the adherence and biofilm formation ofS.epidermidis and M.tuberculosis on the rough surface of titanium alloy and stainless steel were significantly higher than those on the smooth surface (P＜0.05).Conclusions:S.epidermidis and M.tuberculosis isolates had the different adherence and biofilm formation on different biomaterial implants.The adherence and biofilm formation of different bacterial species on different implant surfaces provide the basis for the selection of clinical orthopedic biomaterials.