黄芪丹参不同配伍对气虚血瘀模型大鼠血液流变学和血管内皮因子的影响

摘要

Objective:To study the influence of different compatibility of Astragalus Membranaceus and Salvia Miltiorrhiza on hemorheology and vascular active substances in Qi deficiency and blood stasis rat model.Methods:The SD rats were randomly divided into control group,model group,Salvia miltiorrhiza group,Astragalus membranaceus group,the compatibility of Astragalus Membranaceus and Salvia Miltiorrhiza in different proportion of 1∶1,2∶1,4∶1 groups.The Qi deficiency and blood stasis rats were caused by restricted diet,forced swimming and norepinephrine subcutaneous injection.The control group and model group were given distilled water,the treatment group were given different doses of Salvia miltiorrhiza,Astragalus membranaceus and compatibility of Astragalus Membranaceus and Salvia Miltiorrhiza in different proportion of 1∶1,2∶1 and 4∶1 respectively by gavage for 27 days.The hemorheology and the level of endothelin-1 (ET-1),nitric oxide(NO),thromboxane B2(TXB2) and 6-keto-prostaglandin F1α(6-keto-PGF1α) were determined.Results:Compared with control group,the changes of hemorheology parameters and vascular active substances in the model group were statistically significant (P＜0.01).Compared with model group,the compatibility of Astragalus Membranaceus and Salvia Miltiorrhiza in proportion of 1∶1,2∶1 and 4∶1 groups had significantly lower hemorheology parameters(P＜0.05 or P＜0.01),and the content of NO,6-keto-PGF1α,and 6-keto-PGF1α/TXB2 ratio were significantly higher (P＜0.01),the level of ET-1 and TXB2 was decreased significantly (P＜0.01).Compared with 1∶1 groups,2∶1 groups had significantly lower hemorheology parameters (P＜0.05 or P＜0.01),and the content of NO,6-keto-PGF1 α,and 6-keto-PGF 1 α/TXB2 ratio were significantly higher (P＜0.05 or P＜0.01),the level of TXB2 was decreased significantly (P＜0.05).Compared with 4∶1 group,the changes ofhemorheology parameters and vascular active substances in the 2∶1 group were statistically significant (P＜0.05 or P＜0.01).Conclusions:The compatibility ofAstragalus Membranaceus and Salvia Miltiorrhiza can improve hemorheology,protect vascular endothelium of Qi deficiency and blood stasis rats,and the compatibility of 2∶1 proportion is the best.%目的:研究黄芪丹参不同配伍对气虚血瘀证大鼠血液流变学和血管内皮因子的影响.方法:将SD大鼠随机分为对照组、模型组、丹参组、黄芪组、黄芪丹参1:1组、2:1组、4:1组,采用限食、游泳、皮下注射肾上腺素方法建立气虚血瘀证大鼠模型,对照组与模型组给予蒸馏水,用药组分别给予不同剂量的丹参、黄芪及不同比例黄芪丹参配伍灌胃给药治疗,连续27日.测定血液流变学指标,内皮素-1(ET-1)、一氧化氮(NO)、血栓素B2(TXB2)和6-酮-前列腺素-F1α(6-keto-PGF1α)含量.结果:模型组大鼠血液流变学参数和血管内皮因子与对照组比较,差异具有统计学意义(P＜0.01).与模型组比较,黄芪丹参1:1组、2:1组、4:1组血液流变学参数显著降低(P＜0.05或P＜0.01),NO、6-keto-PGF1α、6-keto-PGF1 α/TXB2明显升高(P＜0.01),ET-1、TXB2明显降低(P＜0.01).与1:1组比较,2:1组血液流变学参数显著下降(P＜0.05或P＜0.01),NO、6-keto-PGF1 α、6-keto-PGF1 α/TXB2显著升高(P＜0.05或P＜0.01),TXB2明显降低(P＜0.05).2:1组大鼠血液流变学参数和血管内皮因子与4:1组比较,差异具有统计学意义(P＜0.05或P＜0.01).结论:黄芪丹参配伍对气虚血瘀证大鼠模型具有改善血液流变及保护血管内皮作用,其中以黄芪丹参2:1组最佳.