Objective:Aim to investigate the effect of gingerol on gastrointestinal function in streptozotocin-induced diabetic rats.Methods:Gingerol was administrated intragastrically at a dose of 100,200 and 400 mg/kg/day to diabetic rats.After 28 dayst,assayed the activity of superoxide dismutase (SOD),catalase (CAT),the content of reduced glutathione (GSH),malondialdehyde (MDA) in gastric mucosa and gastric emptying.Results:The diabetic rats exhibited significant decreases in the above mentioned antioxidative enzymes activities and GSH level and exhibited a high level of MDA(SOD:6.29± 1.03 vs.3.41± 1.21;CAT:27.43± 5.27 vs.10.52± 2.37,GSH:1091.27t 170.09 vs.685.07± 75.24,MDA:9.79± 2.41 vs.46.38± 12.59,P＜0.05).After administration of gingerol,SOD,CAT and GSH level increase (SOD:3.41± 1.21 vs.4.36± 1.01,5.62± 1.18,7.05± 1.48,6.48± 1.82;CAT:10.52± 2.37 vs.15.27± 4.59,19.29± 5.42,23.79± 6.35,GSH:10.52± 2.37 vs.15.27± 4.59,19.29± 5.42,23.79± 6.35,P＜0.05-0.01),MDA level decrease (46.38± 12.59 vs.34.61± 9.27,28.01± 8.34,19.17± 5.19,P＜0.05-0.01);After administration of gingerol,the parameters were ameliorated to a large extent;gingerol treatment dose-dependently augmented the ghrelin levels of stomach and plasma (195.07± 57.42 vs.301.43± 81.24,328.93± 76.59,P＜0.05～0.01),which were earlier depleted in the diabetic control rats;the expression of ghrelin mRNA was decreased after gingerol treatment;the gastric emptying in gingerol treated diabetic rats (45.37± 11.23 vs.52.43± 15.42,49.89± 9.84,74.39± 20.79,P ＜0.05-0.01) was notably accelerated compared with the diabetic control rats.Conclusion:The gingerol can against streptozotocin-induced gastroparesis possibly by its antioxidant property.%目的:探究姜辣素对链霉素诱导糖尿病大鼠胃肠功能的影响.方法:链霉素诱导糖尿病大鼠连续灌胃姜辣素(100,200,400 mg/kg)28天,检测胃部超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性、谷胱甘肽(GSH)和丙二醛(MDA)含量以及胃排空.结果:糖尿病大鼠SOD、CAT以及GSH水平降低(SOD:6.29±1.03 vs.3.41± 1.21;CAT:27.43± 5.27 vs.10.52± 2.37,GSH:1091.27± 170.09 vs.685.07± 75.24,P＜0.05),MDA水平增高(9.79± 2.41 vs.46.38± 12.59,P＜0.05),姜辣素灌胃后SOD、CAT以及GSH水平增高(SOD:3.41± 1.21 vs.4.36± 1.01,5.62± 1.18,7.05± 1.48,6.48± 1.82;CAT:10.52± 2.37 vs.15.27± 4.59,19.29±5.42,23.79± 6.35,GSH:10.52± 2.37 vs.15.27± 4.59,19.29± 5.42,23.79± 6.35,P＜0.05-0.01),MDA水平降低(46.38± 12.59 vs.34.61± 9.27,28.01± 8.34,19.17± 5.19,P＜0.05-0.01);糖尿病大鼠胃ghrelin水平下降(381.26± 94.37 vs.195.07± 57.42,P＜0.01)血浆ghrelin水平上升(76.86± 21.81 vs.108.83± 27.75,P＜0.05)胃ghrelin mRNA表达增多,给予中高剂量姜辣素后胃ghrelin水平上升(195.07± 57.42 vs.301.43± 81.24,328.93± 76.59,P＜0.05～0.01)胃ghrelin mRNA表达减少,呈量效依赖关系;糖尿病大鼠胃排空降低(82.24± 19.74 vs.45.37± 11.23,P＜0.01),给予姜辣素后胃排空增强(45.37± 11.23 vs.52.43± 15.42,49.89± 9.84,74.39± 20.79,P＜0.05-0.01).结论:姜辣素通过抗氧化性改善糖尿病胃轻瘫.
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