电针对糖尿病胃轻瘫大鼠胃动力及胃窦组织Ghrelin和GHSR mRNA表达的影响

摘要

Objective: To explore the action mechanism of electroacupuncture (EA) in improving the gastric motility of rats with diabetic gastroparesis (DGP). Methods: Forty-eight healthy Sprague-Dawley (SD) rats were randomly assigned into four groups: a normal group (group A), a model group (group B), a group of EA at acupoints (group C), and a group of EA at non-acupoints (group D), 12 rats in each group. The animal model of DGP was established by intraperitoneal injection of streptozotocin (STZ) plus high glucose and fat diet. The blood glucose, urine glucose and gastric emptying rate (GER) were observed. The content of insulin (INS) in serum and ghrelin in gastric antrum tissue were detected by enzyme linked immunoassay (ELISA). The expression of growth hormone secretagogue receptor mRNA (GHSR mRNA) in gastric antrum tissue was detected by real-time fluorescent quantitative polymerase chain reaction (PCR). Results:Compared with group A, blood glucose and urine glucose increased significantly (P<0.01), GER, content of serum insulin, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue decreased significantly (P<0.05 orP<0.01) in group B. Compared with group B, blood glucose and urine glucose decreased significantly (P<0.05), GER, the content of insulin in serum, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue increased significantly (P<0.05 orP<0.01) in group C. Conclusion: EA at acupoints can down-regulate the content of blood and urine sugar, and promote gastric emptying, which is possibly related to the regulation of serum insulin, and the expressions of ghrelin and GHSR mRNA in gastric antrum.%目的:探讨电针改善糖尿病胃轻瘫(DGP)大鼠胃动力的机制.方法:将48只健康SD大鼠随机分为4组,即空白对照组(A组)、模型组(B组)、电针穴位组(C组)和电针非穴组(D组),每组12只.采用腹腔注射链脲佐菌素配合高糖高脂饮食建立大鼠DGP模型.观察大鼠血糖、尿糖值和胃排空率,采用酶联免疫法检测血清胰岛素含量、胃窦组织促生长素(ghrelin)含量,实时荧光定量PCR法测定胃窦部生长素促分泌激素受体基因(GHSR mRNA)的表达.结果:与空白组比较,模型组大鼠血糖、尿糖值显著增高(P<0.01),胃排空率、血清胰岛素水平、胃窦ghrelin及GHSR mRNA表达显著降低(P<0.05或P<0.01);经电针穴位后,血糖、尿糖值显著降低(P<0.05),胃排空率、血清胰岛素水平、胃窦ghrelin及GHSR mRNA表达显著增高(P<0.05或P<0.01).结论:电针可降低DGP大鼠血糖、尿糖值,促进胃排空,这可能与其调节血清胰岛素含量,胃窦ghrelin及GHSR mRNA表达有关.