利用均匀设计法设计得到的12种培养基及对照Zarrouk培养基对钝顶螺旋藻(Spirulina platensis)S6品系进行培养,研究了添加磷酸盐的不同培养基下螺旋藻对磷酸盐的吸收利用。结果表明,螺旋藻S6对磷酸盐的净利用量在0.20~0.86mmol/L之间,且与螺旋藻生长速度呈线性关系;磷酸盐为螺旋藻生长利用的主要磷源,含量不足会限制螺旋藻的正常生长;培养基中的磷酸盐添加量以1.16mmol/L左右最为适宜,既可满足藻体的最佳生长,又可降低约60%的磷源养殖成本。%Cultured with twelve media designed with the uniform design method, in comparison with the zarrouk medium,the uptake of phosphate(PO4-P) in different media by the blue-green algae Spirulina platensis strain S6 are studied in this paper. Result shows that the net usage quantity of phosphate by Spirulina S6 is in the range of 0.20~0.86 mmol/L, which has a linear relationship with the algae growth rate. PO4-P is the main phosphorous source of Spirulina S6 growth and usage, and it will limit the growth rate of algae when in a low concentration. The suitable concentration of phosphate is about 1.16 mmol/L, which can maintain the optimum growth of algae and also can reduce the cost of cultivation by 60%.
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