A feeding trial was conducted to evaluate effects of dietary vitamin C on nonspecific immunity of larval and juvenile Symphysodon aequifasciatus with supplement of vitamin C in feed(0~2000.0 mg/kg).When the supplementation ratio reached 500.0 mg/kg(group V)? the alternative complement pathway (ACP) activity,serum lysozyme activity,phagocytosis index(PI) and phagocytosis percentage(PP) of the fish were significantly higher than that of the control (P<0.05),and they increased over time in the same level.And then the non-specific immune index dropped with the increase of dietary vitamin C above 1500.0 mg/kg (group VIII).It was found that proper level of vitamin C supplement in feed may enhance the nonspecific immunity of the larval and juvenile fish,while lack of vitamin C or over-dose may negatively affect the fish health.%通过投喂维生素C含量为0～2 000.0 mg/kg的饲料,对七彩神仙鱼仔幼鱼非特异性免疫进行了研究.结果表明,当饲料中维生素C的添加量达到500.0 mg/kg(V组)以上时,七彩神仙鱼仔幼鱼血清替代补体活力、溶菌酶活力和白细胞吞噬活性均开始显著高于对照组(P＜0.05),且同一水平随时间的增加而升高.添加量达1 500.0 mg/kg(Ⅷ组)之后,随着饲料中维生素C添加量的进一步增加,各组数据反而呈下降趋势.可见饲料中适量添加维生素C能够提高七彩神仙鱼仔幼鱼的非特异性免疫力,维生素C缺乏和过量都会对其带来不利影响.
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