饲料中添加芽孢杆菌PC465对凡纳滨对虾生长和STAT基因表达的影响

摘要

Concentrate liquid of Bacillus firmus PC465 was mixed thoroughly with the feed materials to produce granules at different doses of 106,108 and 1010CFU/g dry matter; or freeze-dried and added to feed at a dose of 107CFU/g dry matter.Three feeding regimes were set up at feeding frequencies of "1 + 1"interval,"4 + 3"interval and continuous feeding.The effects of feeding B.firmus PC465 on growth and expression of STAT gene in Litopenaeus vannamei were studied at different adding doses and feeding frequencies.By adding PC465 at doses of 106,108,and 1010CFU/g,significant improvements were observed in the growth rates and STAT gene expression levels in the lymphoid tissue of L.vannamei (P＜0.05).The effect was the most significant in the continuous feeding treatment.The finding in this research may provide reference to the healthy aquaculture of shrimp.%将坚强芽孢杆菌PC465浓缩茵液直接与饲料原料混匀后制成含106、108和1010 CFU芽孢杆菌/g干物质的颗粒饲料,或者经冷冻干燥制成冻干粉,与饲料原料混匀制成含107 CFU芽孢杆菌/g饲料的颗粒饲料,设连续投喂组、间隔“4+3”投喂组、间隔“1+1”投喂组,研究投喂剂量和投喂频率对对虾生长和类淋巴STAT基因表达的影响.研究发现,饲料中添加不同剂量的坚强芽孢杆菌PC465均能显著提高凡纳滨对虾的生长率和STAT基因表达(P＜0.05),而且跟添加量有一定关系；实验采取不同投喂频率投喂凡纳滨对虾,都能显著提高对虾的生长率和对虾淋巴器官中STAT基因的表达水平(P＜0.05),其中以连续投喂组的效果最明显.研究结果对对虾健康养殖有一定的参考价值.