1,25-二羟维生素D3对哮喘大鼠BALF中Eotaxin及IL-8表达的影响

摘要

目的 观察1,25-(OH)2D3对哮喘大鼠BALF中Eotaxin及IL-8表达的影响.方法 50只Wistar大鼠随机分为正常对照组(N)、哮喘组(A)、1,25-(OH)2D3组(VD)、布地奈德组(B)及1,25-( OH)2D3+布地奈德联合治疗组(L),每组10只.用ELISA法检测BALF中Eotaxin及IL-8的表达水平.结果 各组Eotaxin和IL-8表达比较:A组较其他各组明显增高(P＜0.01、P＜0.05)；VD组较A组明显降低(P＜0.05),但仍高于N组、B组及L组(P＜0.01)；L组较A组、VD组及B组明显减低(P＜0.05、P＜0.01).结论 哮喘急性发作时,BALF中Eotaxin及IL-8的表达水平显著增高,1,25-(OH)2D3有效抑制了哮喘时的Eotaxin和IL-8的表达水平,与布地奈德联合治疗作用更为明显.%Objective To observe the effects of 1,25-(OH)2D3 on the levels of eotaxin and IL-8 in BALF of asthmatic rats. Methods SO Wistar rats were randomly divided into five groups:Normal control group(N) .asthma group(A) ,1,25-(OH)2D3 group(VD) ,budesonide group(B) ,and therapeutic alliance group of 1,25-(OH)2D3 and budesonide ( L) ,10 rats for each group. The expressive levels of eotaxin and IL-8 in BALF were detected by ELISA. Results The levels of eotaxin and IL-8 increased more significantly in group A than those of other groups( P <0. 01, P < 0. 05 ) ; the levels in group VD decreased markedly compared with group A ( P < 0. 05 ) , but remarkably higher than group N, group B and group L(P<0. 01); the levels of group L decreased more markedly than those of group A, group B and group VD( P < 0. 05 ,P < 0. 01). Conclusion The levels of eotaxin and IL-8 in BALF of acute episodes of asthma significantly increased. 1,25-(OH)2D3 can efficiently inhibit the levels of eotaxin and IL-8 in asthma,and the combination of 1,25-( OH)2D3 and budesonide has a more significant effect.