目的 观察1,25二羟维生素D3(VD)对哮喘大鼠气道重塑及其肺组织中纤溶酶原激活物抑制剂-1( PAI-1)表达和血浆中PAI-1含量的影响.方法 30只健康雄性Wistar大鼠随机分为对照组、哮喘组、VD干预组,每组各10只.卵蛋白致敏和激发复制慢性哮喘模型.VD干预组每次激发前给予VD 干预.用免疫组化检测肺组织PAI-1的表达,酶联免疫法测血浆中PAI-1含量,采用图像分析进行图像分析.结果 (1)哮喘组支气管管壁厚度较对照组和VD干预组显著增加(P＜0.01).(2)哮喘组PAI-I在大鼠肺组织的表达程度较对照组和VD干预组显著增加(P＜0.01).(3)哮喘大鼠血浆中PAI-1含量较对照组和VD干预组明显增加(P＜0.01).(4)直线相关性分析显示,哮喘组支气管管壁厚度与大鼠肺组织中PAI-1表达水平呈正相关(r=0.822,P＜0.01)；哮喘组支气管管壁厚度与大鼠血浆中PAI-1含量呈正相关(r=0.942,P＜0.01).结论 1,25二羟维生素D3干预可明显减轻慢性哮喘气道重塑的病理改变,并可通过部分抑制PAI-1的表达来延缓气道重塑.%Objective To observe the effects of 1 ,25 ( OH) 2D, on the airway remodeling and the expression of PAI-1 in asthma rats. Methods Eighteen wistar rats were randomly divided into three group;the control group, the asthma model group, and the 1, 25 ( OH ) 2 D, group. The asthma model was established by sensitization and challenged with ovalbumin,and the rats in the 1,25(OH)2D, group injected 1,25(OH)2D3 before the challenge. The following parameters were measured; the protein expression of PAI -1 by immunohistochemistry and the level of PAI -1 by ELISA. The bronchial wall thickness by computer-assisted image analysis system. Results (1 ) the bronchial wall thickness in the asthma group was higher than in the other groups(P <0. 01). (2) The expression of PAI -1 in the asthma group higher than that in other groups(P <0. 01). (3)The lever of PAI-1 in plasma of asthma group was higher than that in other groups(P <0. 01). (4) There was a positive correlation between the activity of PAI-1 in rat lungs and the bronchial wall thickness( r =0. 822 P < 0. 01 ) ,the same between the level of PAI-1 in plasma and the bronchial wall thickness( r =0. 942 P <0. 01) Conclusion 1,25 ( OH) 2 D3 could decrease the expression of PAI-1 in lung tissues of asthmatic rats and the level of PAI-1 in plasma and inhibit airway remodeling.
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