Objective To establish a method for the content determination of aminophylline and morphine in kechuanning mixture. Methods The determination was performed on Shisheido C8 column with the mobile phases of 0.1 mol/L potassium dihydrogen phosphate solution-0.005 mol/L sodium heptanesulfonate solution-acetonitrile (47. 5:47. 5:5). The detection wavelength for aminophylline was 271 nm and the sample volume was 10 μL;the detec-tion wavelength for morphine was 207 nm and the sample volume was 20 μL. Results The linear range of the theo-phylline was 8. 62~68. 96 μg/mL with an average recovery of 99. 2%;The linear range of the morphine was 1. 03~51. 60 μg/mL with an average recovery of 101. 5%. Conclusion The method is accurate,precise and specific for the quality control of aminophylline and morphine in kechuanning mixture.%目的 建立咳喘宁合剂中氨茶碱和吗啡含量测定的HPLC方法. 方法 采用高效液相色谱法,色谱柱为资生堂C8 色谱柱,流动相为0.1 mol/L 磷酸二氢钾溶液 -0.005 mol/L 庚烷磺酸钠水溶液 -乙腈(47. 5:47. 5:5). 氨茶碱检测波长为271 nm,进样量为10 μL;吗啡检测波长为207 nm,进样量为20 μL. 结果茶碱在8. 62~68. 96 μg/mL范围内具有良好的线性关系,平均回收率为99. 2%;吗啡在1. 03 ~51. 60 μg/mL范围内具有良好的线性关系,平均回收率为101. 5%. 结论 该方法具有良好的准确度、精密度、专属性,能良好地控制咳喘宁合剂的质量.
展开▼
