葛根素对人胰腺癌PANC-1细胞增殖凋亡的影响

摘要

目的 探讨葛根素对人胰腺癌细胞株PANC-1的作用及其机制.方法 取对数生长期的PANC-1细胞,分别用不同浓度的葛根素(0、50、100、200 μmol/L)干预48 h后,利用CCK-8检测PANC-1细胞增殖水平;Annexinv-FITC/PI双染流式细胞术检测细胞凋亡;Western blot检测Fas、Fas-L、Bax、Bcl-2、Caspase-7表达水平.结果 葛根素对胰腺癌PANC-1细胞增殖的抑制呈浓度依赖性;不同浓度的葛根素干预PANC-1细胞48 h后,细胞凋亡率分别为2.47％±0.64％、6.37％±0.71％、8.33％±0.90％、12.40％±1.01％,实验组与对照组相比,差异均有统计学意义(P ＜0.01);Western blot实验表明,葛根素对胰腺癌PANC-1细胞干预48 h后,随着葛根素浓度的增加,细胞中Fas、Fas-L、Caspase-7、Bax蛋白的表达量上调,Bcl-2的表达量下调,实验组与对照组相比,差异均有统计学意义(P＜0.05).结论 葛根素可通过激活Fas/Fas-L信号通路诱导PANC-1细胞增殖抑制和凋亡.%Objective To investigate the effect and mechanism of puerarin on human pancreatic cancer cell line PANC-1.Methods PANC-1 cells of logarithmic growth phase were treated with different concentrations of puerarin (0,50,100 and 200 μmol/L) for 48 h;CCK-8 was used to detect the proliferation level of PANC-1 cells.Apoptosis was detected by Annexinv-FITC/PI double staining flow cytometry.Western blot was used to detect the expression of Fas,Fas-L,Bax,Bcl-2 and Caspase-7.Results Puerarin inhibited the proliferation of pancreatic cancer PANC-1 cells in a concentration-dependent manner.When different concentrations of puerarin interfered PANC-1 cells for 48 h,the apoptosis rate of cells respectively was 2.47％ ±0.64％,6.37％ ±0.71％,8.33％ ±0.90％,and 12.40％ ± 1.01％,the difference between experimental group and control group being statistically significant (P ＜ 0.01).Western blot experiment showed that,after the 48 h intervention for PANC-1 of pancreatic cancer cell line,the expression of Fas,FasL,Caspase-7 and Bax protein increased with the increase of puerarin concentration,while the expression of Bcl-2 protein decreased,the difference between experimental group and control group being statistically significant (P ＜ 0.05).Conclusion Puerarin can induce PANC-1 cell proliferation inhibition;Apoptosis by activating Fas/Fas-L signaling pathway.