Objective To observe the effect of Aloes burn cream on peripheral serum inflammatory cytokines and foot ulcer in diabetic rats.Methods Viripotent Wistar rats (n =120) were chosen to establish the diabetes model,and 100 successful model rats were randomized into peripheral circulatory disorders group (n =50) and foot ulcer group (n =50).Forty-two rats in peripheral circulatory disorders group and 40 rats in food ulcer group were randomly assigned to control group (silver sulfadiazine) and intervention group (silver sulfadiazine + aloes burn cream) respectively.The plantar temperature and hot pain threshold of rats were detected in peripheral circulatory disorders group at 1,2 and 3 weeks after treatment;meanwhile,the recovery of ulcer,level of VEGF and inflammatory factor,including CRP,TNF-α and IL-6 in serum,were detected in foot ulcer group.Results The plantar temperature of rats decreased in peripheral circulatory disorders group during the first 2 weeks' treatment (P ＜ 0.05);the plantar temperature was higher in intervention group after treatment,which was also higher than that of control group (P ＜ 0.05).There was no significant difference in the pain threshold between the two peripheral circulatory disorders groups before treatment (P ＞ 0.05);the pain threshold decreased gradually in both groups after treatment (P ＜ 0.05),and there were significantdifferences between the two groups (P ＜ 0.05).The recovery rate of foot ulcer and the level of VEGF in foot ulcer rats of intervention group were higher than those of control group (P ＜ 0.05),but the levels of inflammatory factor (VEGF,TNF-α and IL-6) in intervention group were lower (P ＜ 0.05).Conclusion Silver sulfadiazine combined with Aloes bum cream can improve the plantar temperature and pain dysesthesia of rats caused by diabetic peripheral circulatory disorders,and can promote the wound healing of diabetic foot ulcers in rats by up-regulating the VEGF and down-regulating the inflammatory factor.%目的 研究芦荟烧伤膏对糖尿病大鼠足溃疡的保护作用,并研究其对外周血清炎性因子的影响.方法 将性成熟Wistar大鼠120只(造模成功100只)制成糖尿病大鼠模型,将成功造模的100只大鼠随机分为2组(末梢循环障碍模型组、足溃疡模型组),每组50只.将成功造模的末梢循环障碍模型组(42只)与足溃疡模型组(40只)随机分为干预组与对照组,两组大鼠均给予磺胺嘧啶银涂抹患处,干预组在此基础上给予芦荟烧伤膏,连续给药35 d.治疗1、2、3周记录末梢循环障碍大鼠足底皮温、疼痛阈值,观察足溃疡大鼠溃疡面愈合情况,记录外周血清血管内皮生长因子(VEGF)及其炎性因子的浓度.结果 治疗后的前2周,两组末梢循环障碍模型大鼠的足底温度均降低(P＜0.05),治疗后第3周,干预组皮温升高(P＜0.05),且干预组皮温高于对照组(P＜0.05);治疗前,两组大鼠痛阈比较差异无统计学意义(P＞0.05),治疗后,两组大鼠痛阈逐渐降低(P＜0.05),且干预组低于对照组(P＜0.05).治疗后2、3周,两组足溃疡模型大鼠中,干预组的愈合率及VEGF水平高于对照组(P＜0.05),但其炎性因子(CRP、TNF-α、IL-6)水平低于对照组(P＜0.05).结论 在磺胺嘧啶银的基础上给予芦荟烧伤膏,能改善糖尿病大鼠末梢循环引起的温度及痛觉迟钝,同时能通过提高VEGF的表达与抑制炎性因子的释放,促进足溃疡大鼠溃疡面愈合,值得临床推广.
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