miR-409-5p对肝癌细胞系HepG2增殖及迁移的影响

摘要

Objective The objective of this study was to observe effects of miR-409-5p on the proliferation and migration of HepG2 cells.Methods HepG2 cells were transfected with miR-409-5p mimics and verified by Real-Time quantitative PCR.After high expression of miR-409-5p,MTT and wound healing assays were used to detect the proliferation and migration ability of HepG2 cells.Results The cell viability of HepG2 cells transfected with miR-409-5p mimics was significantly decreased in comparison with the control group and showed a dose-and time-dependent manner(P<0.05).The migration ability of cells overexpressing miR-409-5p mimics was significantly lower than that in the NC group(P<0.05).There was significant difference between the two groups at treatments for 24 h and 48 h.Conclusion Up-regulated miR-409-5p can inhibit the proliferation and migration of HepG2 cells.%目的 观察过表达miR-409-5p对肝癌细胞系HepG2增殖及迁移能力的影响.方法 利用miR-409-5p mimics进行转染肝癌细胞系HepG2,并通过实时荧光定量PCR进行验证.高表达miR-409-5p后,使用MTT法和划痕实验分别检测HepG2细胞增殖能力及迁移能力的变化.结果 MTT实验显示,HepG2细胞转染miR-409-5p mimics后细胞增长趋势减缓,且随miR-409-5p mimics浓度和时间成正比.划痕实验显示,在24h和48h两个时间点三组间比较差异均有统计学意义(P<0.05),证实过表达miR-409-5p mimics的细胞体外迁移能力明显低于转染阴性对照组(NC mimics)组和对照组.结论 上调miR-409-5p可抑制肝癌细胞系HepG2的增殖及迁移能力.