菟丝子总多糖对家兔全层关节软骨缺损Ⅱ型胶原表达的影响

摘要

Objective To discussion about the effect of total polysaccharide from Cuscuta chinensis Lam on rabbits' full-thickness articular cartilage defects in the expression of type Ⅱ collagen. Methods We divide 72 healthy adult Japanese white rabbits into 3 groups randomly: the total polysaccharide from Cuscuta chinensis Lam group ( group A ) , the compounds of bone morphogenetic protein and gelatin group ( group B ) and the blank control group ( group C ). There were 24 rabbits including 48 knee joints in every group. Full-thickness articular cartilage defect area with diameter of 3mm and depth of about 3nn were created in trochlear surface in the medial femoral condyle of the knee joint with crank drill. For group A and group B,the defect area was filled with total polysaccharide from Cuscuta chinensis Lam and the compounds of bone morphogenetic protein and gelatin respectively. Group C was left without any treatment. We executed the experimental animals in batch at the end of 2,4,8 weeks after operation and evaluated? by histology observation and immunohistochemistry method for type Ⅱ collagen. Results Repair tissues were dominated by hyaline cartilage according to histology observation. Compared with group C ,the expression of type Ⅱ collagen in group A and group B were significantly different at the end of 2,4,8 weeks after operation ( P ＜ 0. 01 ). Conclusion Rabbits' full-thickness articular cartilage defects can be repaired with total polysaccharide from Cuscuta chinensis Lam.%目的 探讨中药提取物菟丝子总多糖局部运用对家兔关节软骨全层缺损Ⅱ型胶原表达的影响.方法 将72只成年日本大耳白兔分为菟丝子总多糖组、人骨形态发生蛋白(BMP)/明胶复合物组、空白对照组,每组24只,48个关节.在膝关节股骨内髁滑车面上,用手摇钻造成直径3 mm、深约3 mm软骨全层缺损区,分别给予菟丝子总多糖凝胶、BMP/明胶复合物填充;空白对照组只做钻孔处理,空白对照.术后第2、4、8周分批处死实验动物,采用组织学观察及Ⅱ型胶原免疫组化的方法对Ⅱ型胶原的表达进行评估.结果 经组织学观察修复组织以透明软骨为主,接近正常关节软骨;用药组Ⅱ型胶原的表达在术后2、4、8周与空白组比较,差异均有极显著统计学意义(P＜0.01).结论 菟丝子总多糖局部运用可以修复家兔关节软骨缺损,可能是通过促进Ⅱ型胶原的表达来实现的.