甲状腺转录因子及Napsin A在肺腺癌转移至浆膜腔积液脱落细胞中的表达

摘要

目的：探讨甲状腺转录因子（thyroid transcription factor-1，TTF-1）及Napsin A在肺腺癌浆膜腔积液脱落细胞学中的诊断价值。方法收集胸水及心包积液标本67例，经离心沉淀制作成细胞石蜡块，经HE切片筛检出肺腺癌转移42例、间皮细胞反应性增生16例及乳腺癌转移9例。采用免疫组织化学SP法标记三组细胞中TTF-1、Napsin A的表达。结果TTF-1在肺腺癌中表达38例（90.48％），在增生间皮细胞及乳腺癌细胞中无表达；Napsin A在肺腺癌中表达35例（83.33％），在增生间皮细胞中表达仅1例（6.25％），肺腺癌中TTF 及Napsin A的表达明显高于其他两组，差异有统计学意义（ P ＜0.001）。结论 TTF-1和Napsin A是两项重要的肺腺癌标记物，对鉴别浆膜腔积液脱落细胞学的组织来源具有重要价值。%Objective To investigate the diagnostic value of thyroid transcription factor-1(TTF-1) and Napsin A for identifi-cation of pulmonary adenocarcinoma cells in pleural effusions.Methods The samples of pleural effusions and pericardial effusions were collected from 67 patients and made to be paraffin cytoblocks after precipitation by centrifugation.From these samples,42 cases were identified as lung adenocarcinoma,16 cases as mesothelial cell reactive hyperplasia and 9 cases as breast adenocarcinoma by using HE staining.Then,expressions of TTF-1 and Napsin A in the three types of diseases were examined with immunohistochemical SP meth-od.Results TTF-1 expression was positive in 38 cases with pulmonary adenocarcinoma but negative in cases with hyperplasia mesothe-lial cells and breast adenocarcinomas.Napsin A expression was positive in 35 cases with pulmonary adenocarcinoma and one case with hyperplasia of mesothelial cells.The differences were all significant ( P <0.001 ) .Conclusion These data suggest that TTF-1 and Napsin A are two important markers for the pulmonary adenocarcinoma.They are very usefully to identify the cells in pleural effusions.