白介素-17对人宫颈腺癌细胞株HeLa体外增殖的影响

摘要

Objective To explore the effect of IL-17 on proliferation of human cervical adenocarcinoma cell line HeLa in vitro and the underlying mechanisms of the effect.Methods First,human cervical adenocarcinoma cell line HeLa was stimulated with varying concentrations of rIL-17 (0,1,10,50 to 100 ng/ml) for 24 hours.The proliferation rate was detected by a MTT assay.Then,following experiments were carried out according to the selected concentrations of rIL-17.The induced apoptosis cells by starvation were stimulated with rIL-17 (0,50 and 100 ng/ml) for 24 h,the apoptosis was detected by flow cytometry.RT-PCR and Western blot were used to detect the expression of VEGF at mRNA and protein levels after 48 h of cell stimulation by rIL-17.Results After stimulation by rIL-17 for a certain period of time,cells proliferation in the 1,l0and 100 ng/ml concentration groups of rIL-17 had no significant changes,but proliferation rate in the 50 ng/ml group was increased significantly (P ＜ 0.01).The lateapoptosis rate and total apoptosis rate in 50 ng/ml group were significantly decreased when compared with the normal control group (P ＜ 0.01 and ＜ 0.05,respectively),and the late apoptosis and total apoptosis rate of cells in the 100 ng/ml group were decreased more significantly (all P ＜ 0.01).The production of VEGF mRNA and protein in both the 50 ng/ml and 100 ng/ml,especially in 50 ng/ml group,were increased when compared with the control group (P ＜ 0.05).Conclusion IL-17 could promote the proliferation of cervical adenocarcinoma cell line HeLa in vitro by inhibiting apoptosis and up-regulating the production of VEGF mRNA and protein.%目的 观察IL-17对人宫颈腺癌细胞株HeLa细胞体外增殖的影响,并探讨可能的机制.方法 分别采用0、1、10、50、100 ng/ml的rIL-17刺激人宫颈腺癌HeLa细胞24小时,MTT法检测细胞增殖情况,根据筛选出的浓度进行后续实验.用rIL-17(0、50、100 ng/ml)刺激经饥饿诱导凋亡的细胞24小时,FCM法检测细胞凋亡情况;用rIL-17刺激细胞48小时,检测促血管生成因子VEGF mRNA和蛋白的表达量.结果 rIL-17刺激细胞一定时间后,1、10及100 ng/ml浓度组细胞增殖无明显变化,50 ng/ml组细胞增殖明显增加(P＜ 0.01);50 ng/ml组细胞的晚期凋亡率和总凋亡率较正常对照组均明显下降(P＜0.01;P＜ 0.05),100 ng/rnl组细胞下降更为显著(P＜0.01);50 ng/ml和100 ng/ml两组细胞内VEGF mRNA和蛋白的表达量均升高,以50 ng/ml组最为明显(P＜0.05).结论 IL-17可能通过抑制细胞凋亡及上调VEGF mRNA和蛋白水平的表达促进宫颈腺癌HeLa细胞体外增殖.